FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_88.3520000005c816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_88.3520000005c816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31147
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA4971.5956592930298263No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA2650.850804250810672No Hit
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2260.7255915497479692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.5201142967219957No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1520.4880084759366873No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA1310.4205862522875397No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1000.32105820785308375No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA960.3082158795389604No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA930.2985841333033679No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA930.2985841333033679No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA900.28895238706777543No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA890.28574180498924456No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA840.26968889459659035No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA830.2664783125180595No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA830.2664783125180595No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA780.2504254021254053No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA770.24721482004687448No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA750.2407936558898128No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA730.23437249173275115No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC730.23437249173275115No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA720.23116190965422032No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA710.22795132757568948No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA700.22474074549715864No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA690.2215301634186278No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA670.21510899926156613No Hit
GTGAATGGGGTCAAGCTCAAAAAAAAAAAAAAA620.19905608886891193No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA590.18942434263331942No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA580.18621376055478858No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA520.16695026808360355No Hit
AGCTCAACACGTACTCAAAAAAAAAAAAAAAAA520.16695026808360355No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA490.15731852184801104No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA470.15089735769094936No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.14768677561241852No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA450.14447619353388771No Hit
GTGAATGGGGTCAAGCTAAAAAAAAAAAAAAAA440.14126561145535688No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC440.14126561145535688No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.14126561145535688No Hit
CTCAACACGTACTCAAAAAAAAAAAAAAAAAAA430.13805502937682604No Hit
AACGCAGAGTGAATGGGTCAAGCTCAACACGTA430.13805502937682604No Hit
GCTCAACACGTACTAAAAAAAAAAAAAAAAAAA400.1284232831412335No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA380.12200211898417182No Hit
AGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.11879153690564098No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC370.11879153690564098No Hit
GAGTGAATGGGGTCAAGCTCAAAAAAAAAAAAA360.11558095482711016No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC360.11558095482711016No Hit
GTATCAACGCAGAGTGAATGGGGTCAAGCTCAA350.11237037274857932No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA340.10915979067004848No Hit
ACGCAGAGTGAATGGGGTCAAGCTCAACACGTA330.10594920859151764No Hit
ACGCAGAGTGAATGGGTCAAGCAAAAAAAAAAA330.10594920859151764No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACAAAA320.1027386265129868No Hit
ACGCAGAGTGAATGGGGTCAAGCTCAAAAAAAA320.1027386265129868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC257.5876235E-427.0418681
ATCTACG257.6595804E-426.9983942
TCTACGC257.6595804E-426.9983943
AACGTCA200.00848691126.9983926
ACACTTA200.00848691126.99839222
AACACTT200.00848691126.99839221
TACGCAG300.00221658122.4986615
CTACGCA300.00221658122.4986614
TATCAAC2300.017.0481341
TGCGTTG1258.0035534E-1116.19903627
TCTGCGT4500.015.89905427
ATCAACG2650.014.7727052
TCAACGC2900.013.4991963
CTGCGTT1705.857146E-1012.70512626
CTCTGCG5950.012.02449326
ATGGGGA959.6783537E-411.36774355
CAACGCA3550.011.0275134
ACTCTGC6500.011.00703625
GTATCAA2253.5106495E-1010.8167481
ACGTCAA1153.9529725E-410.5645887
Produced by FastQC (version 0.11.2)