FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_86.3520000005c7d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_86.3520000005c7d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20943
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT3521.6807525187413457No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2651.2653392541660697No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA2351.1220933008642506No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA1950.931098696461825No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC1950.931098696461825No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA1740.8308265291505514No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA1560.74487895716946No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC1330.6350570596380652No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1280.611182734087762No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA1240.5920832736475194No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA1200.5729838132072769No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA1150.5491094876569738No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA1130.5395597574368525No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA1100.5252351621066704No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1070.5109105667764886No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA1060.506135701666428No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA1000.4774865110060641No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG960.4583870505658215No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA910.4345127250155183No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA910.4345127250155183No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA890.424962994795397No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC860.41063839946521513No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA800.38198920880485127No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA790.3772143436947906No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA790.3772143436947906No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC690.32946569259418423No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA670.31991596237406295No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA640.305591367043881No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA630.30081650193382037No Hit
GTGAATGGGGTTGCCAAAAAAAAAAAAAAAAAA610.2912667717136991No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA600.28649190660363844No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA600.28649190660363844No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA580.27694217638351715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.26739244616339586No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA550.2626175810533352No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA540.25784271594327457No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA540.25784271594327457No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA510.2435181206130927No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA510.2435181206130927No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA500.23874325550303205No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA490.2339683903929714No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA490.2339683903929714No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA480.22919352528291076No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA480.22919352528291076No Hit
GCAACAACGTACTCTGAAAAAAAAAAAAAAAAA450.21486892995272885No Hit
GTTGTTGCCAAAAAAAAAAAAAAAAAAAAAAAA450.21486892995272885No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC440.2100940648426682No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA430.20531919973260757No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA420.20054433462254692No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA420.20054433462254692No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA410.19576946951248628No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA410.19576946951248628No Hit
GTGAATGGGTACGTTGTTGCCAAAAAAAAAAAA400.19099460440242563No Hit
GCAACAACGTACTCTGCGAAAAAAAAAAAAAAA400.19099460440242563No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA390.186219739292365No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA390.186219739292365No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.18144487418230434No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA370.1766700090722437No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA350.16712027885212244No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC340.1623454137420618No Hit
GGCAACAACGTACTCTGAAAAAAAAAAAAAAAA340.1623454137420618No Hit
GTGAATGGGCAACAACGTACTCTGCGTAAAAAA330.15757054863200115No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCAA320.1527956835219405No Hit
ACGCAGAGTGAATGGGGTTGCCAAAAAAAAAAA320.1527956835219405No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT300.14324595330181922No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG300.14324595330181922No Hit
GTGTTCGGCAAAAAAAAAAAAAAAAAAAAAAAA300.14324595330181922No Hit
GCAACAACGTACTCTAAAAAAAAAAAAAAAAAA290.13847108819175857No Hit
GAGTACGTTGTTGCCAAAAAAAAAAAAAAAAAA290.13847108819175857No Hit
CAACGTACTCTAAAAAAAAAAAAAAAAAAAAAA290.13847108819175857No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.13847108819175857No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA290.13847108819175857No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA290.13847108819175857No Hit
CAACAACGTACTCTGCGTAAAAAAAAAAAAAAA280.13369622308169793No Hit
GGCAACAACGTACTCTAAAAAAAAAAAAAAAAA280.13369622308169793No Hit
GTGAATGGGTTGCCAAAAAAAAAAAAAAAAAAA280.13369622308169793No Hit
GTGAATGGGGAGTACGTTGTTGCCAAAAAAAAA260.12414649286157665No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.12414649286157665No Hit
ACGCAGAGTGAATGGGGTTGTTGCCAAAAAAAA260.12414649286157665No Hit
GCAACAACGTACTCTGCGTAAAAAAAAAAAAAA260.12414649286157665No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA250.11937162775151602No Hit
ACAACGTACTCTGCGTTAAAAAAAAAAAAAAAA250.11937162775151602No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA250.11937162775151602No Hit
ACGTACTCTAAAAAAAAAAAAAAAAAAAAAAAA240.11459676264145538No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11459676264145538No Hit
GAATGGGCAACAACGTACTCAAAAAAAAAAAAA240.11459676264145538No Hit
GAGTGAATGGGCAACAACGTACTCTGCGAAAAA230.10982189753139474No Hit
TATCAACGCAGAGTGAATGGGGTTGCCAAAAAA230.10982189753139474No Hit
GTACGTTGTTGCCAAAAAAAAAAAAAAAAAAAA230.10982189753139474No Hit
GAGTGAATGGGCAACAACGTACTCTGCGTAAAA230.10982189753139474No Hit
GAGTGAATGGGGTTGTTGCCAAAAAAAAAAAAA230.10982189753139474No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA220.1050470324213341No Hit
ACGTACTCAAAAAAAAAAAAAAAAAAAAAAAAA220.1050470324213341No Hit
AACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA220.1050470324213341No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA210.10027216731127346No Hit
GGCAACAACGTACTCTGCGTAAAAAAAAAAAAA210.10027216731127346No Hit
ACGTACTCTGAAAAAAAAAAAAAAAAAAAAAAA210.10027216731127346No Hit
GGGCAACAACGTACTCAAAAAAAAAAAAAAAAA210.10027216731127346No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA210.10027216731127346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1052.217348E-916.7142851
TGCGTTG1054.126923E-815.42857127
TCTGCGT3600.013.527
CTGCGTT1305.6135286E-712.46153826
ATCAACG1502.4211113E-711.72
TCAACGC1553.706009E-711.322583
GCTGTTC900.00830187210.57
CAACGCA1853.6127803E-69.4864864
CCTGTTC1802.7926418E-48.258
GTGAATG5550.08.0270271
TGAATGG5450.07.92660572
CTCTGCG6250.07.77626
CGCAGAG3151.0506119E-77.7142862
AACGCAG2305.6426004E-57.63043455
ACGCAGA3201.3702993E-77.593751
ACTCAAA1600.0077643357.5937510
CTGTTCG3951.2769306E-97.5189879
CAGAGTG3158.9519926E-77.28571464
GCAGAGT3158.9519926E-77.28571463
CGGCAAC6700.07.253731313
Produced by FastQC (version 0.11.2)