FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_84.3520000005c79e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_84.3520000005c79e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62352
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC2680.4298178085706954No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.3464203233256351No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA1350.2165127020785219No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA1350.2165127020785219No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1320.2117013086989992No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.19405953297408263No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.13953040800615857No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1315114190402874No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA800.12830382345393893No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT740.11868103669489352No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA680.10905824993584809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG307.0721595E-526.9887642
CTGAGTG307.0721595E-526.9887644
ACGCTGA307.0721595E-526.9887641
GAGTGAC200.00856647726.98876411
GTATCAT505.0695235E-926.9887641
TATCTAC307.0721595E-526.9887641
GCTGAGT307.0721595E-526.9887643
TGAGTGA307.0721595E-526.9887645
TATCATC551.39862095E-824.5352382
CAACTCA352.0147601E-423.1332261
ATCATCG707.66704E-921.2054583
TCATCGC707.66704E-921.2054584
CATCGCA707.66704E-921.2054585
GGTACTG454.528073E-520.991266
ATCGCAG658.21492E-820.7605886
TGCCACT404.9684173E-420.2415735
TCGCAGA701.7949606E-719.2776897
TCTGCGT17150.017.63947327
GTACTGT1601.6370905E-1114.3377817
GCGTTGA2000.014.18048227
Produced by FastQC (version 0.11.2)