FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_83.3520000005c774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_83.3520000005c774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63610
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC4910.7718912120735734No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.7027196981606665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.5282188335167426No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.4747681182204056No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1260.1980820625687785No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA960.15091966671906934No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.1462034271340984No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA820.1289105486558717No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT790.12419430907090079No Hit
GTGAATGGGAGTCCAAAAAAAAAAAAAAAAAAA760.11947806948592989No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA720.113189750039302No Hit
CCCCCAGTCCTTCTGTGACGTACTCTGCGTTGA710.11161767017764503No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA690.10847351045433107No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC670.10532935073101714No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA660.10375727086936017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT604.3655746E-1127.01
CAACACA200.00855037327.01
CAACTCA601.2860255E-924.751
GTAGAGT352.0097061E-423.1428594
TATCATC653.246896E-922.8461552
ATCATCG653.246896E-922.8461553
TCATCGC653.246896E-922.8461554
TAGAGTG300.002241328822.55
AACGTAG553.8033977E-722.090911
ACGTAGA553.8033977E-722.090912
ATACCAC1050.021.85714327
CGTAGAG454.5154113E-521.03
TACGCAG454.5154113E-521.05
TCGCAGA658.1852704E-820.7692327
ATCGCAG658.1852704E-820.7692326
CATCGCA658.1852704E-820.7692325
ACGCTGA404.956E-420.251
TCACAGT350.00547673719.2857155
ATCTACG501.0153188E-418.9000022
TCTACGC501.0153188E-418.9000023
Produced by FastQC (version 0.11.2)