FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_82.3520000005c75b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_82.3520000005c75b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21425
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA1400.6534422403733956No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA930.43407234539089845No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA930.43407234539089845No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA870.4060676779463244No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA790.3687281213535589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.308051341890315No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.29404900816802804No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA630.29404900816802804No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA610.2847141190198366No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA570.2660443407234539No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA530.24737456242707118No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA510.23803967327887982No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA510.23803967327887982No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.2333722287047841No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA480.22403733955659277No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA480.22403733955659277No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA470.2193698949824971No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA440.20536756126021002No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA420.19603267211201866No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA380.17736289381563594No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA380.17736289381563594No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA380.17736289381563594No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA370.17269544924154026No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA370.17269544924154026No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA360.16802800466744458No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAG350.1633605600933489No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.15869311551925322No Hit
GTATCATCGCAGAGTGAATGGGATCGAGACGAG330.1540256709451575No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA330.1540256709451575No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA320.14935822637106186No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA300.14002333722287047No Hit
GTGAATGGGGATCGAGACAAAAAAAAAAAAAAA280.1306884480746791No Hit
AGTACGTACAAAAAAAAAAAAAAAAAAAAAAAA280.1306884480746791No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC270.12602100350058343No Hit
TATCTACGCAGAGTGAATGGGATCGAGACAAAA260.12135355892648775No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA260.12135355892648775No Hit
ACGCAGAGTGAATGGGATCGAGACGAAAAAAAA260.12135355892648775No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT260.12135355892648775No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA260.12135355892648775No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGT260.12135355892648775No Hit
CAACTCAGAGTGAATGGGATCGAGACGAGAAAA260.12135355892648775No Hit
GACGAGTACGTACAAAAAAAAAAAAAAAAAAAA260.12135355892648775No Hit
GAATGGGACGAGTACAAAAAAAAAAAAAAAAAA250.11668611435239205No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACAAA240.11201866977829639No Hit
GAGTGAATGGGACGAGTACGAAAAAAAAAAAAA240.11201866977829639No Hit
GATCGAGACGAGAAAAAAAAAAAAAAAAAAAAA240.11201866977829639No Hit
AACGCAGAGTGAATGGGATCGAGACGAGAAAAA230.1073512252042007No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC230.1073512252042007No Hit
ACGCAGAGTGAATGGGACGAGTACGAAAAAAAA230.1073512252042007No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT220.10268378063010501No Hit
GTGAATGGGACGAGTAAAAAAAAAAAAAAAAAA220.10268378063010501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG200.00842620527.06
CAACTCA405.625461E-727.01
AGTAAAT200.00842620527.02
GTAAATG200.00842620527.03
GTATCAT306.841129E-526.9999981
ATCTACG451.5717742E-624.0000022
TCTACGC451.5717742E-624.0000023
TATCTAC451.5717742E-624.0000021
CTACGCA451.5717742E-624.0000024
TATCATC351.9496225E-423.1428592
TACGCAG503.927089E-621.5999985
AACTCAG558.963372E-619.6363642
ACTCAGA558.963372E-619.6363643
TCAGAGT558.963372E-619.6363645
CTCAGAG558.963372E-619.6363644
GTATCAA1400.019.2857151
TCGCAGA450.001062603118.0000027
ATCGCAG450.001062603118.0000026
TCATCGC450.001062603118.0000024
CATCGCA450.001062603118.0000025
Produced by FastQC (version 0.11.2)