FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_81.3520000005c724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_81.3520000005c724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45429
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2120.4666622641924762No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1520.3345880384776244No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1500.33018556428712936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.3235818530013868No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC1280.2817583481916837No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1220.26855092562019856No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1160.2553435030487134No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA1130.2487397917629708No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC1070.2355323691914856No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1060.2333311320962381No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA1010.22232494662000044No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC1010.22232494662000044No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA990.21792247242950535No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA950.20911752404851527No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA920.20251381276277267No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA860.1893063901912875No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA850.18710515309603998No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT790.17389773052455482No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA790.17389773052455482No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA780.17169649342930726No Hit
GTGAATGGGAGTACGTAACGGAAAAAAAAAAAA770.16949525633405974No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1672940192388122No Hit
GAGTACGTAACGGAAAAAAAAAAAAAAAAAAAA750.16509278214356468No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.14748288538158444No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA600.13207422571485175No Hit
GTGAATGGGGAGTACGTAACGGAAAAAAAAAAA590.12987298861960422No Hit
GGAGTACGTAACGAAAAAAAAAAAAAAAAAAAA550.12106804023861409No Hit
GTGAATGGGGTACGTAACGAAAAAAAAAAAAAA530.11666556604811905No Hit
GTGAATGGGGTACGTAACGGAAAAAAAAAAAAA490.10786061766712893No Hit
CCCCCAGCCATTCCGTTACGTACTCTGCGTTGA480.10565938057188139No Hit
GCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.10565938057188139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.008525073527.01
CGCAAAG200.008525073527.02
GCGTTGA3900.020.07692327
TATCAAC3350.014.1044791
TGCGTTG7600.012.96710527
TCAACGC4050.011.3333333
ATCAACG4050.011.3333332
CTGCGTT8950.011.01117326
CAACGCA4400.010.4318184
GTATCAA3500.010.0285721
TCAGCCA1751.9583167E-610.0285725
TCTGCGT13850.09.45487427
ATGGGGC1350.00175357499.0000015
AACGCAG5500.08.8363635
TTAGCCA1608.5891696E-48.43757
CTCTGCG16200.08.08333326
AAGCCAT4201.0913936E-118.0357148
CTAGCCA2203.5555022E-57.9772734
ACGCAGA8050.07.8819881
ACTCTGC17400.07.60344825
Produced by FastQC (version 0.11.2)