FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_80.3520000005c717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_80.3520000005c717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41409
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC5211.2581805887608974No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA5111.2340312492453331No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA5011.2098819097297688No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA3690.8911106281243207No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.8862807602212079No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA3660.8838658262696515No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA3340.806587939819846No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA2920.705160713854476No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT2260.5457750730517521No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.5337004032939698No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA2180.5264556014393006No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA2170.5240406674877442No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA2120.5119659977299621No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA2030.4902315921659543No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1870.45159264894105144No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1800.43468811128015644No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA1650.39846410200681015No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1630.3936342341036973No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1550.37431476249124584No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1500.36224009273346375No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA1410.3405056871694559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.3380907532178995No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1400.3380907532178995No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.3308459513632302No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA1270.306696611847666No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA1270.306696611847666No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1230.29703687604144025No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1150.27771740442898885No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA1090.2632278007196503No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA1040.2511531309618682No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA1000.2414933951556425No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.23183365934941683No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA950.22941872539786037No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA940.22700379144630395No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC940.22700379144630395No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG910.21975898959163465No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.21734405564007822No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA870.21009925378540897No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC860.20768431983385255No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC850.2052693858822961No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA840.20285445193073967No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC800.193194716124514No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC790.19077978217295757No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA790.19077978217295757No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA790.19077978217295757No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA780.18836484822140115No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA770.18594991426984472No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA730.176290178463619No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA710.17146031056050617No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA690.16663044265739332No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA670.16180057475428047No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA660.15938564080272405No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA660.15938564080272405No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA650.15697070685116762No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA620.14972590499649835No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA620.14972590499649835No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA600.1448960370933855No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.14248110314182907No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA580.14006616919027265No Hit
CCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.13765123523871622No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA560.1352363012871598No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA550.13282136733560337No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA550.13282136733560337No Hit
GGGCTAACTATCCACGTACTCAAAAAAAAAAAA520.1255765654809341No Hit
GAATGGGCTAACTATCCACGTACTCAAAAAAAA520.1255765654809341No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA510.12316163152937767No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA490.11833176362626482No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA490.11833176362626482No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA490.11833176362626482No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA480.11591682967470841No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA470.11350189572315197No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA460.11108696177159555No Hit
GTGAATGGGCTAACTATCCACGTAAAAAAAAAA460.11108696177159555No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1062570938684827No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10384215991692627No Hit
GAGTGAATGGGGCTAACTATCCAAAAAAAAAAA420.10142722596536984No Hit
CTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.10142722596536984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.0085164127.01
GTATCAT800.027.01
AACTCAG200.0085164127.02
TCAGAGT200.0085164127.05
CTCAGAG200.0085164127.04
TATCATC850.025.4117622
TCGCAGA900.024.07
ATCATCG900.024.03
ATCGCAG900.024.06
TCATCGC950.022.7368434
CATCGCA950.022.7368435
AACGTAG300.002228174422.51
GAGAATG350.00544501219.28571513
ATCTACG350.00544501219.2857152
TCTACGC350.00544501219.2857153
AGAATGG350.00544501219.28571514
TACGCAG350.00544501219.2857155
TATCTAC350.00544501219.2857151
CTACGCA350.00544501219.2857154
CAGAGAG501.00441066E-418.9000029
Produced by FastQC (version 0.11.2)