FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_79.3520000005c6fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_79.3520000005c6fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45790
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA2050.4476960034942127No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA2000.43677658877484166No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA1930.4214894081677222No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1670.3647084516269928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.3385018563005023No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1510.3297663245250055No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1460.3188469098056344No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1250.2729853679842761No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA1160.2533304214894082No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1110.2424110067700371No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1070.2336754749945403No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA1040.22712382616291768No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA900.19654946494867875No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA870.18999781611705613No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA850.1856300502293077No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA840.1834461672854335No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA820.17907840139768508No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA810.17689451845381088No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA800.17471063550993665No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA790.17252675256606245No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA760.16597510373443983No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA750.16379122079056563No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA700.15287180607119458No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA640.13976850840794935No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT640.13976850840794935No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT600.1310329766324525No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA590.1288490936885783No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA580.1266652107447041No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA550.12011356191308147No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA550.12011356191308147No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA550.12011356191308147No Hit
CATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA530.11574579602533304No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA510.11137803013758464No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.11137803013758464No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA500.10919414719371041No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA500.10919414719371041No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA480.104826381305962No Hit
GTGAATGGGATTGCGATAAAAAAAAAAAAAAAA480.104826381305962No Hit
GACATCGCAAAAAAAAAAAAAAAAAAAAAAAAA460.10045861541821358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTTA200.008527433526.99890922
TCGCAGA405.870461E-726.9989097
ATCGCAG405.870461E-726.9989096
GACTTAC200.008527433526.99890923
GTATCAT451.6240374E-624.0252861
TATCATC451.6396334E-623.9990272
ATCATCG451.6396334E-623.9990273
TCATCGC451.6396334E-623.9990274
CAACTCA351.9834777E-423.1672381
AACTCAG351.998275E-423.1419222
TAGAGTG300.00223231522.499095
TCAGAGT404.928133E-420.2491825
CTCAGAG404.928133E-420.2491824
ACTCAGA500.00220414816.1993433
GTATCAA2250.014.4151731
TCTGCGT6600.014.31760227
TATCAAC3150.013.2997121
ATCAACG3650.011.4652882
TCAACGC3600.011.2495443
TGCGTTG2202.6375346E-1011.04500827
Produced by FastQC (version 0.11.2)