FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_78.3520000005c6d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_78.3520000005c6d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45909
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA2730.594654642880481No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2080.45307020409941406No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1730.37683242937114725No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA1660.3615848744254939No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.27445598902176044No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1250.2722777668866671No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA1130.24613910126554706No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1040.22653510204970703No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA990.21564399137424034No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA950.20693110283386698No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT920.20039643642858698No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA790.17207954867237363No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA770.16772310440218693No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA750.16336666013200027No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA750.16336666013200027No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA740.16118843799690694No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA670.14594088305125355No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA660.14376266091616022No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA610.13287155024069355No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC610.13287155024069355No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA600.13069332810560022No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA590.1285151059705069No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA570.12415866170032021No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC550.11980221743013353No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA550.11980221743013353No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.11108932888976017No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA500.10891110675466684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG200.00853408426.9945324
ACGCTGA200.00853408426.9945321
TACGCAG200.00853408426.9945325
TATCTAC200.00853408426.9945321
CTACGCA200.00853408426.9945324
TGAGTTC200.00853408426.9945326
GTGAATA257.7218574E-426.994531
TGAATAG300.00223438322.4954412
CCTGTTC404.9334107E-420.24597
TCTGCGT6600.014.3309627
TATCAAC1704.3655746E-1113.4972651
ACGTTCG750.00219816612.5974478
ATCAACG1851.7644197E-1012.4028922
TCAACGC1902.764864E-1012.07653
GATACCA906.1959005E-412.0107127
TGGGTTC2750.011.7794316
ATGGGTT2650.011.7146075
AATGGGT3350.011.6841994
TGCGTTG2451.8189894E-1211.58175627
TGATACC959.754303E-411.37856726
Produced by FastQC (version 0.11.2)