FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_7.3520000005bdf8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_7.3520000005bdf8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76636
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA5360.6994101988621535No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA5080.66287384519025No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA4550.5937157471684326No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA3740.4880212954747116No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA3400.44365572315882873No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA3360.43843624406284254No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA3200.4175583276788976No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG3060.39929015084294583No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA2980.38885119265097345No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA2910.37971710423299754No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA2570.33535153191711464No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA2420.31577848530716635No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA2080.2714129129912835No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA1940.2531447361553317No Hit
GTGAATGGGGCATTGCTGAAGACAAAAAAAAAA1940.2531447361553317No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC1880.2453155175113524No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA1800.23487655931937992No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA1680.21921812203142127No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC1630.21269377316143848No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA1620.21138890338744193No Hit
GTGAATGGGCATTGCTGAAGACGAAAAAAAAAA1610.21008403361344538No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA1580.2061694242914557No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA1580.2061694242914557No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA1510.19703533587347982No Hit
GTATCATCGCAGAGTGAATGGGCATTGCTGAAG1470.1918158567774936No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA1420.18529150790751084No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG1410.1839866381335143No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA1400.18268176835951772No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA1390.18137689858552117No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA1380.18007202881152462No Hit
GCTGAAGACGAAAAAAAAAAAAAAAAAAAAAAA1370.17876715903752805No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1360.1774622892635315No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA1330.17354767994154183No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA1310.17093794039354873No Hit
TATCTACGCAGAGTGAATGGGCATTGCTGAAGA1250.1631087217495694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.16180385197557282No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA1240.16180385197557282No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAA1110.1448405449136176No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA1080.14092593559162794No Hit
GAATGGGCATTGCTGAAAAAAAAAAAAAAAAAA1070.1396210658176314No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.13831619604363485No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGAAA1000.1304869773996555No Hit
GCATTGCTGAAGACGAAAAAAAAAAAAAAAAAA1000.1304869773996555No Hit
GTATCAACGCAGAGTGAATGGGGCATTGCTGAA950.12396262852967274No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA940.1226577587556762No Hit
GAGTGAATGGGCATTGCTGAAGACAAAAAAAAA900.11743827965968996No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA890.11613340988569341No Hit
GAATGGGCATTGCTGAAGAAAAAAAAAAAAAAA880.11482854011169687No Hit
GCATTGCTGAAGACGTAAAAAAAAAAAAAAAAA860.11221880056370373No Hit
CCCCCCGCATTGCTGAAGACGTACTCTGCGTTG850.11091393078970718No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA820.10699932146771751No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA820.10699932146771751No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA810.10569445169372096No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAAAA800.1043895819197244No Hit
CCCCCGCATTGCTGAAGACGTACTCTGCGTTGA780.10177984237173131No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA780.10177984237173131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGG200.00854889927.0078533
CGGACTC200.00854889927.00785322
ATTAACG200.00854889927.0078533
TTAACGC200.00854889927.0078534
GTGTATG200.00857625626.9901891
CAACTCA950.026.9901891
AAGACGG257.777263E-426.99018918
AGACGGA257.777263E-426.99018919
TGTATGG200.00857625626.9901892
GTATCAT800.025.3033031
TATCATC800.025.3033032
TATCTAC651.200533E-1024.9140221
ATCTACG601.3005774E-924.7410092
TCTACGC653.261448E-922.85283
AACTCAG1150.022.2962442
CTACGCA707.674316E-921.2204554
GCTGCAT454.5207E-521.0061076
TCATCGC1050.020.5774144
TACGCAG801.6043487E-920.255895
TCGCAGA1003.6379788E-1220.255897
Produced by FastQC (version 0.11.2)