FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_68.3520000005c583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_68.3520000005c583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49453
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGGAGACTACGTACTCTGCGTTGATACCA4570.9241097607829657No Hit
GTAGGAGACTACGTACTCTGCGTTGATACCACT4530.9160212727235961No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA2460.4974420156512244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.48935352759185485No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA2270.4590216973692192No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1640.3316280104341496No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1610.3255616443896225No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.3053404242411987No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.27096434998887836No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA1240.2507431298404546No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC1220.24669888581076985No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.24669888581076985No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA1090.2204112996178189No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA880.17794673730612906No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA870.17592461529128667No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA860.1739024932764443No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA800.16176976118739006No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA780.1577255171577053No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA740.1496370290983358No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA740.1496370290983358No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA710.14357066305380867No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA680.13750429700928155No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA630.12739368693506967No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA600.12132732089054253No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA580.11728307686085777No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA550.11121671081633065No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA530.1071724667866459No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA510.10312822275696115No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC500.10110610074211877No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.10110610074211877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT554.620233E-1027.01
TATCATC554.620233E-1027.02
TCACACG200.00853215927.05
TATCTAC551.3775207E-824.5454541
CAACTCA501.5017213E-724.31
TCTACGC501.5017213E-724.33
TACGCAG501.5017213E-724.35
CTACGCA501.5017213E-724.34
ATCTACG603.4691766E-822.52
TCAGAGT608.7002627E-720.255
ATCATCG751.6669219E-819.83
TCATCGC751.6669219E-819.84
TACCACT559.364985E-619.63636427
CATCGCA701.7682942E-719.2857135
AACTCAG651.8738028E-618.6923072
ACTCAGA651.8738028E-618.6923073
CTCAGAG651.8738028E-618.6923074
TATCAAC2700.016.51
TCGCAGA757.3342617E-616.1999997
ATCGCAG757.3342617E-616.1999996
Produced by FastQC (version 0.11.2)