FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_67.3520000005c569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_67.3520000005c569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40208
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5291.3156585754078791No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC3920.9749303621169917No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA3670.9127536808595303No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA3260.8107839235972941No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA2760.6864305610823717No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA2520.6267409470752089No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA2470.6143056108237167No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA2390.594409072821329No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA2290.5695384003183446No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC2160.5372065260644648No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA1990.49492638280939116No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA1950.4849781138081974No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA1940.48249104655789893No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1800.4476721050537207No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA1790.4451850378034222No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA1730.43026263430163153No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA1710.42528849980103467No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA1680.4178272980501393No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1610.40041782729805014No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1590.3954436927974533No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA1580.3929566255471548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.3680859530441703No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA1470.36559888579387184No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA1460.3631118185435734No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA1350.33575407879029046No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA1300.3233187425387982No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT1290.3208316752884998No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA1260.31337047353760444No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA1240.3083963390370076No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA1210.3009351372861122No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC1170.29098686828491843No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA1150.2860127337843215No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1130.28103859928372465No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA1030.25616792678074013No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG1000.2487067250298448No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA990.24621965777954635No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA960.23875845602865103No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.2312972542777557No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA920.22881018702745723No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA920.22881018702745723No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA880.21886191802626345No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA860.21388778352566654No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA860.21388778352566654No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA850.2114007162753681No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA850.2114007162753681No Hit
CTGAACGTACTCAAAAAAAAAAAAAAAAAAAAA850.2114007162753681No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA800.19896538002387587No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA780.19399124552327895No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1915041782729805No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.1865300437723836No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA720.17906884202148826No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA670.16663350576999603No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT660.16414643851969757No Hit
GAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA660.16414643851969757No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA660.16414643851969757No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA660.16414643851969757No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.15917230401910068No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA640.15917230401910068No Hit
TCAACGCAGAGTGAATGGGCTCTGAACGTACTC640.15917230401910068No Hit
ACGCAGAGTGAATGGGCTCTGAACGTAAAAAAA600.14922403501790688No Hit
CAACGCAGAGTGAATGGGCTCTGAACAAAAAAA570.14176283326701153No Hit
CAACTCAGAGTGAATGGGCTCTGAACGTACTCT570.14176283326701153No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA550.13678869876641464No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTAAAAA550.13678869876641464No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA550.13678869876641464No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA550.13678869876641464No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA540.1343016315161162No Hit
GAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA530.13181456426581775No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA530.13181456426581775No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA520.1293274970155193No Hit
CTGAACGTACTCTGCAAAAAAAAAAAAAAAAAA520.1293274970155193No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA520.1293274970155193No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA510.12684042976522086No Hit
AACGTAGAGTGAATGGGCTCTGAACGTACTCTG510.12684042976522086No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA510.12684042976522086No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA480.11937922801432552No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.11937922801432552No Hit
GTGAATGGGAATGCGCAAAAAAAAAAAAAAAAA480.11937922801432552No Hit
GTGAATGGGCTCTGAACGTACTCTGCGAAAAAA440.10943095901313173No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA440.10943095901313173No Hit
CAACGCAGAGTGAATGGGCTCTGAACGTACTCA430.10694389176283327No Hit
GCTCTGAACGTACTCTGCGTAAAAAAAAAAAAA430.10694389176283327No Hit
ACGCAGAGTGAATGGGCTCAAAAAAAAAAAAAA430.10694389176283327No Hit
CCCCCAATGCGCTCTGAACGTACTCTGCGTTGA430.10694389176283327No Hit
CAACGCAGAGTGAATGGGCTCTGAACGTACTCT420.10445682451253482No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.10445682451253482No Hit
GAACGTACTCTGCGTTAAAAAAAAAAAAAAAAA420.10445682451253482No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA410.10196975726223638No Hit
CAACGCAGAGTGAATGGGCTCTGAACGAAAAAA410.10196975726223638No Hit
GAATGGGCTCTGAACAAAAAAAAAAAAAAAAAA410.10196975726223638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGT200.00850776227.0037363
GTATCAT455.4184056E-826.970151
TATCTAC700.026.9701481
TATCATC405.8951446E-726.9701482
CGCATAG200.00855969126.9701482
TCTACGC751.8189894E-1225.2034873
TACGCAG751.8189894E-1225.2034875
CTACGCA751.8189894E-1225.2034874
ATCTACG751.8189894E-1225.1721382
CGCTGAG401.812091E-523.598882
ACGCTGA401.812091E-523.598881
CTGAGTG351.9899159E-423.1460594
GCTGAGT351.9899159E-423.1460593
TGAGTGA351.9899159E-423.1460595
ATCATCG559.282385E-619.6390823
TCATCGC601.965596E-518.0024914
CATCGCA601.965596E-518.0024915
GTAAATG500.002195181816.2022423
GAGTAAA500.00221357316.182091
AGTAAAT500.00221357316.182092
Produced by FastQC (version 0.11.2)