FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_63.3520000005c4e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_63.3520000005c4e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47770
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC2530.5296211011094829No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.44588653966924846No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2100.4396064475612309No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1290.2700439606447561No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1290.2700439606447561No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1280.26795059660875026No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1220.2553904123927151No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1160.24283022817667993No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA1050.21980322378061545No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1040.21770985974460957No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA970.20305631149256856No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA800.16746912288046892No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA740.15490893866443375No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.15281557462842787No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT690.14444211848440444No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC680.1423487544483986No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA660.13816202637638686No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA620.1297885702323634No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1297885702323634No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT520.10885492987230479No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.10885492987230479No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.10885492987230479No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT480.10048147372828135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGCA200.00852942827.011
GAGCAAC200.00852942827.013
TCGCAGA356.410508E-627.07
ATCATCG356.410508E-627.03
ATCGCAG356.410508E-627.06
TCATCGC356.410508E-627.04
GTATCAT401.8058865E-523.6251
CATCGCA401.8058865E-523.6255
CGCTGAG300.002233215122.52
CTGAGTG300.002233215122.54
ACGCTGA300.002233215122.51
CTTCGTT300.002233215122.524
TCTTCGT300.002233215122.523
TGAGTGA300.002233215122.55
CTCTTCG350.005457169419.28571522
GCTGAGT350.005457169419.2857153
TATCATC552.0928269E-417.1818182
TCTGCGT11650.016.22317527
CGTTGAT2500.016.227
TATCAAC2800.014.9464291
Produced by FastQC (version 0.11.2)