FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_60.3520000005c484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_60.3520000005c484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56470
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA5490.9721976270586152No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA5430.961572516380379No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA5290.9367805914644944No Hit
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5140.9102178147689038No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA4170.7384451921374181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.6693819727288826No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC3720.6587568620506463No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA3380.5985479015406411No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA2310.4090667611120949No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA1990.3523995041615017No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT1980.35062865238179564No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA1860.32937843102532316No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA1820.322295023906499No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.3063573578891447No Hit
GGATTCACGTACTCTGCGAAAAAAAAAAAAAAA1610.2851071365326722No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA1600.2833362847529662No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA1590.28156543297326014No Hit
GATTCACGTACTCTGCGAAAAAAAAAAAAAAAA1510.2673986187356118No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA1500.2656277669559058No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG1460.2585443598370816No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA1430.2532318044979635No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA1430.2532318044979635No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA1410.24969010093855143No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA1350.23906499026031522No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.23729413848060918No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.23552328670090314No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.23198158314149106No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA1300.23021073136178502No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA1240.21958562068354878No Hit
GTGAATGGGCGTAAGGATTCACGTACTCAAAAA1220.2160439171241367No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.21250221356472462No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA1090.19302284398795821No Hit
ATCAACGCAGAGTGAATGGGATTCACGTACTCT1080.19125199220825218No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA1070.18948114042854614No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGT1040.18416858508942802No Hit
ATTCACGTACTCTGCGTAAAAAAAAAAAAAAAA1030.18239773330972198No Hit
ACGCAGAGTGAATGGGCGTAAGGATTCACGTAC1020.18062688153001594No Hit
GGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA950.16823091907207366No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA900.1593766601735435No Hit
GTGAATGGGATTCACGTACTCTGCGTAAAAAAA890.15760580839383745No Hit
GGATTCACGTACTCTGCGTAAAAAAAAAAAAAA880.1558349566141314No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA860.15229325305471933No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA850.1505224012750133No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA820.14520984593589517No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA820.14520984593589517No Hit
GATTCACGTACTCTGCGTAAAAAAAAAAAAAAA800.1416681423764831No Hit
CTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAA790.13989729059677705No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA790.13989729059677705No Hit
CTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.138126438817071No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA750.1328138834779529No Hit
GAATGGGATTCACGTACTCTGCGAAAAAAAAAA750.1328138834779529No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA730.1292721799185408No Hit
GGTATCAACGCAGAGTGAATGGGATTCACGTAC720.12750132813883477No Hit
GAGTGAATGGGATTCACGTACTCTGCGTAAAAA710.12573047635912873No Hit
CTCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAA700.12395962457942271No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC700.12395962457942271No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.12218877279971667No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA690.12218877279971667No Hit
GTGAATGGGATTCACGTACTCAAAAAAAAAAAA680.12041792102001063No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA670.11864706924030459No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA660.11687621746059855No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA630.11156366212148043No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA600.10625110678236231No Hit
ACGCAGAGTGAATGGGATTCACGTACTCAAAAA580.10270940322295023No Hit
GTATCATCGCAGAGTGAATGGGATTCACGTACT570.10093855144324419No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA570.10093855144324419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA200.00854240627.0000025
GTATTAA200.00854240627.0000021
ATTAACG200.00854240627.0000023
TCAGAGA200.00854240627.0000025
TATTAAC200.00854240627.0000022
AACGTAG257.735443E-426.9999981
TTAACGC257.735443E-426.9999984
GTATCAT751.8189894E-1225.1999991
TATCATC751.8189894E-1225.1999992
CAACTCA701.0913936E-1125.0714281
GTATGGG401.8135E-523.6250023
GTGTATG401.8135E-523.6250021
TGTATGG401.8135E-523.6250022
ACTCAGA752.7284841E-1123.43
CTCAGAG752.7284841E-1123.44
TCACGCG352.0058276E-423.1428595
AACTCAG806.548362E-1121.9375022
TCAGAGT608.746192E-720.255
CGCTGAG559.404423E-619.6363622
CTGAGTG559.404423E-619.6363624
Produced by FastQC (version 0.11.2)