FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_59.3520000005c46a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_59.3520000005c46a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58214
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA4090.7025801353626275No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA3490.5995121448448827No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC2470.4242965609647164No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA2430.4174253615968668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.3555845672862198No Hit
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA1970.33840656886659565No Hit
ACGCAGAGTGAATGGGGCACTTACGTACTCAAA1690.29030817329164804No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.2679767753461367No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1490.25595217645239976No Hit
GGCACTTACGTACTCAAAAAAAAAAAAAAAAAA1490.25595217645239976No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA1200.20613598103548975No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA1190.20441818119352734No Hit
GTATCAACGCAGAGTGAATGGGGCACTTACGTA1150.19754698182567768No Hit
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.19582918198371527No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA1110.190675782457828No Hit
GAGTGAATGGGGCACTTACGTACTCAAAAAAAA1080.18552238293194076No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA1050.18036898340605353No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA1030.1769333837221287No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA960.1649087848283918No Hit
GGACTGAGGCACAAAAAAAAAAAAAAAAAAAAA920.15803758546054214No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA920.15803758546054214No Hit
GTATCATCGCAGAGTGAATGGGGCACTTACGTA910.15631978561857973No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.15116638609269248No Hit
GGGCACTTACGTACTCAAAAAAAAAAAAAAAAA830.1425773868828804No Hit
GTGAATGGGGCACTTACGTACAAAAAAAAAAAA790.13570618751503075No Hit
CTTACGTACTCTGCGAAAAAAAAAAAAAAAAAA780.13398838767306834No Hit
GAATGGGACTGAGGCACAAAAAAAAAAAAAAAA780.13398838767306834No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC710.12196378877933144No Hit
GAACTGAGGCACAAAAAAAAAAAAAAAAAAAAA700.12024598893736903No Hit
GTGAATGGGGACTGAGGCAAAAAAAAAAAAAAA700.12024598893736903No Hit
GAACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA690.1185281890954066No Hit
AACGCAGAGTGAATGGGGCACTTACGTACTCAA690.1185281890954066No Hit
ATCAACGCAGAGTGAATGGGGCACTTACGTACT680.1168103892534442No Hit
TATCTACGCAGAGTGAATGGGGCACTTACGTAC680.1168103892534442No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA670.11509258941148177No Hit
ACGCAGAGTGAATGGGGCACTTACGTACTCTGC610.1047857903597073No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA600.10306799051774487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT200.00850706427.02415826
CTTACTC307.0010115E-527.02415825
CGGCACT200.00854298527.000866
GTATCAT455.4920747E-826.9776041
TATCATC501.510798E-724.3007762
AGCACTG501.510798E-724.3007766
CAACTCA850.023.803771
CTTACTT352.0063206E-423.14359521
TACTTAC352.0063206E-423.14359523
AACTCAG900.022.5007172
GAGTAAA300.00224982522.4813391
ATCTACG454.50554E-521.000672
TCTACGC454.50554E-521.000673
TACGCAG454.50554E-521.000675
CTACGCA454.50554E-521.000674
TATCTAC454.535647E-520.982581
GTATCAA1650.020.437581
ACTCAGA1003.6379788E-1220.2506473
TCAGAGT953.092282E-1119.8953725
TAAATGG350.00547006319.286334
Produced by FastQC (version 0.11.2)