FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_57.3520000005c427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_57.3520000005c427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41711
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC3190.7647862674114742No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1430.342835223322385No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1390.33324542686581476No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1340.32125818129510203No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1300.3116683848385318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.2948862410395339No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1140.273309199012251No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1050.251732156984968No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC940.22536021672939993No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA850.20378317470211696No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA850.20378317470211696No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA770.1846035817889765No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA710.17021888710412122No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT700.16782143798997867No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA630.15103929419098078No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA610.1462443959626957No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA610.1462443959626957No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA580.13905204862026804No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA560.13425715039198294No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA540.12946225216369783No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA490.11747500659298506No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA490.11747500659298506No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA470.11268010836469997No Hit
GGCTACGTACCGTAAAAAAAAAAAAAAAAAAAA440.10548776102227231No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA430.10309031190812974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC200.00851722327.03
TAACCTA200.00851722327.05
TACGCAG200.00851722327.05
GTATCAT451.6332233E-624.01
TATCATC451.6332233E-624.02
AACCTAC401.7987424E-523.6256
ATCATCG559.307707E-619.6363643
TCATCGC651.8595601E-618.6923074
TCGCAGA601.9709229E-518.07
ATCGCAG601.9709229E-518.06
CATCGCA601.9709229E-518.05
TGATACC1258.367351E-1116.227
AATGGGT851.3412428E-615.8823524
TCTGCGT9000.014.8527
TTGATAC1404.656613E-1014.46428626
TATCAAC2050.013.8292691
GCCCTAC800.00355175411.81257
ATGGGGG800.00355175411.81255
GTTGATA1801.9777872E-811.2525
CTCTACG1659.126579E-710.6363648
Produced by FastQC (version 0.11.2)