FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_56.3520000005c41a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_56.3520000005c41a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48550
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA10832.2306900102986615No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA3880.7991761071060761No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3560.733264675592173No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA3080.6343975283213182No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC2670.5499485066941298No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA2500.5149330587023687No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA2380.490216271884655No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA2250.46343975283213185No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA2070.4263645726055613No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2040.42018537590113286No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA2000.4119464469618949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.33779608650875387No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA1640.33779608650875387No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC1620.3336766220391349No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1380.2842430484037075No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA1280.26364572605561276No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA1130.23274974253347067No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA1110.2286302780638517No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA1060.21833161688980435No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA1050.21627188465499483No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA1020.21009268795056643No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA990.203913491246138No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA930.19155509783728117No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA870.1791967044284243No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC850.17507723995880534No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA840.17301750772399588No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA830.1709577754891864No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA830.1709577754891864No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA800.164778578784758No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC760.1565396498455201No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG680.1400617919670443No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA670.1380020597322348No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA670.1380020597322348No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA650.13388259526261587No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA650.13388259526261587No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA640.13182286302780638No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA620.12770339855818744No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA580.11946446961894953No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT580.11946446961894953No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA580.11946446961894953No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC580.11946446961894953No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC560.11534500514933058No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA560.11534500514933058No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA550.11328527291452112No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT530.10916580844490217No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA520.10710607621009269No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC520.10710607621009269No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA510.10504634397528322No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA500.10298661174047373No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA500.10298661174047373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT257.719603E-427.04
AACGTAG257.719603E-427.01
CAACTCA257.719603E-427.01
ACGTAGA257.719603E-427.02
CGTAGAG257.719603E-427.03
TACGACA200.00853073127.02
TAGAGTG307.0204216E-527.05
CTACGCA451.6424692E-624.0000024
ATCTACG401.8066497E-523.6252
TCTACGC401.8066497E-523.6253
TACGCAG401.8066497E-523.6255
TATCTAC401.8066497E-523.6251
GTATCAT504.102176E-621.61
TCATCGC608.69386E-720.254
CATCGCA608.69386E-720.255
CTACTCT350.005458384819.2857154
GTACGAC350.005458384819.2857151
ATCGCAG501.0090026E-418.9000026
ATCATCG651.8724368E-618.6923083
GATACCA1900.018.47368427
Produced by FastQC (version 0.11.2)