FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_55.3520000005c3e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_55.3520000005c3e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72134
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA3910.5420467463332133No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA3720.5157068788643357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.5087753347935786No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA2750.3812349238916461No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA2630.3645992181218288No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC2580.3576676740510716No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG2270.314692100812377No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA2210.3063742479274683No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA2030.2814206892727424No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA1700.23567249840574486No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA1610.2231957190783819No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1570.21765048382177613No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA1520.21071893975101894No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA1470.20378739568026175No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA1470.20378739568026175No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA1330.18437907228214157No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA1240.1719022929547786No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC1200.16635705769817286No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA1190.1649707488840214No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA1100.15249396955665845No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA1010.14001719022929549No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA1000.13863088141514404No Hit
GCATTGCTGAAGACGTACTCAAAAAAAAAAAAA990.1372445726009926No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA970.1344719549726897No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA950.13169933734438682No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA940.1303130285302354No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA940.1303130285302354No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA920.12754041090193252No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT900.12476779327362963No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA900.12476779327362963No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA890.12338148445947819No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA850.11783624920287243No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA840.116449940388721No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA840.116449940388721No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG810.11229101394626667No Hit
GCATTGCTGAAGACGTACAAAAAAAAAAAAAAA800.11090470513211523No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA760.10535946987550947No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA760.10535946987550947No Hit
GTGAATGGGGCATTGCTGAAGACAAAAAAAAAA750.10397316106135802No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA740.1025868522472066No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA740.1025868522472066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT653.6379788E-1227.0083271
CAACTCA405.9321064E-727.0083261
AACATCG200.00854498827.0083263
TATCATC701.0913936E-1125.079162
AACTCAG451.6566792E-624.0074022
CTCAGAG451.6566792E-624.0074024
ATCATCG801.8189894E-1223.6322863
TCATCGC752.7284841E-1123.4072174
GTAGAGT352.0091468E-423.1499944
AACGTAG352.0091468E-423.1499941
ACGTAGA352.0091468E-423.1499942
CGTAGAG352.0091468E-423.1499943
ACTCAGA504.1373078E-621.6066633
TCGCAGA901.4551915E-1120.9919097
ATCGCAG901.4551915E-1120.9919096
CATCGCA1250.020.5120945
TAGAGTG404.977848E-420.2421975
CATGCAT404.977848E-420.2421976
AATGCAT559.495718E-619.6287986
ACATCGC350.0054739619.2916624
Produced by FastQC (version 0.11.2)