FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_53.3520000005c3af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_53.3520000005c3af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29946
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG1660.5543311293661926No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1370.45749014893474926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.42743605155947373No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA870.2905229412943298No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT850.283844252988713No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA840.2805049088359046No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA750.25045081146062914No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA700.23375409069658717No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA610.2036999933213117No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA570.19034261671007816No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC540.180324584251653No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA520.17364589594603622No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA490.16362786348761105No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA470.15694917518199425No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC450.15027048687637748No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA440.14693114272356908No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA430.1435917985707607No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA430.1435917985707607No Hit
GACGTAGCTCACGTACTCAAAAAAAAAAAAAAA380.12689507780671874No Hit
GTGAATGGGTAGACGTAGCTCAAAAAAAAAAAA350.11687704534829359No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.11687704534829359No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT340.11353770119548522No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT330.11019835704267683No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGCTC330.11019835704267683No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA330.11019835704267683No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA320.10685901288986843No Hit
ACGCAGAGTGAATGGGTAGACGTAGCTCAAAAA320.10685901288986843No Hit
ACGCAGAGTGAATGGGTAGACGTAAAAAAAAAA310.10351966873706005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT356.3300013E-626.9866221
TATCATC356.3300013E-626.9866222
TCGCAGA401.783462E-523.6132957
ATCATCG401.783462E-523.6132953
ATCGCAG401.783462E-523.6132956
TCATCGC401.783462E-523.6132954
CATCGCA401.783462E-523.6132955
AACTCAG351.9818501E-423.131392
ACTCAGA351.9818501E-423.131393
TCAGAGT351.9818501E-423.131395
CTCAGAG351.9818501E-423.131394
CAACTCA404.888171E-420.2399671
TATCAAC2500.015.1125091
CGTTGAT2150.015.08753127
ATCAACG2750.013.2479782
TCAACGC2950.012.3498113
GTATCAA1604.5813067E-811.8066471
CAACGCA3150.011.5656954
GCGTTGA2650.011.20199426
AATGGGC1607.2150015E-610.1199844
Produced by FastQC (version 0.11.2)