FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_50.3520000005c35f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_50.3520000005c35f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66611
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.4969149239615079No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2120.31826575190283884No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1030.15462911531128493No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1020.1531278617645734No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA1010.15162660821786192No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA1000.1501253546711504No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT940.14111783339088138No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA890.13361156565732385No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC830.12460404437705484No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA810.12160153728363182No Hit
GCTCACTACCACAAAAAAAAAAAAAAAAAAAAA780.11709777664349731No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA760.11409526955007432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA200.008543842527.006021
TCTACGC200.008543842527.006023
ATCATCG352.0080178E-423.1480183
TCATCGC352.0080178E-423.1480184
CATCGCA352.0080178E-423.1480185
TCGCAGA352.0286247E-423.1132347
ATCGCAG352.0286247E-423.1132346
TACGCAG300.00223949722.5050185
GTATCAT454.511653E-521.004681
TATCATC454.511653E-521.004682
GTTGATA1351.6370905E-1116.00356727
TATCAAC2350.014.3649051
TCTGCGT13950.013.55140827
CGTTGAT2201.8189894E-1212.27546227
TGCGTTG6100.012.17484527
ATCAACG2550.011.6496562
TCAACGC2600.011.4256243
ATGGGGG1452.3383636E-611.1749055
GCGTTGA3500.010.80240727
CTGCGTT6900.010.76326826
Produced by FastQC (version 0.11.2)