FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_5.3520000005bda8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_5.3520000005bda8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36369
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG2290.6296571255739778No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.4234375429624131No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC870.23921471582941514No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA800.21996755478566912No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC760.20896917704638565No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA730.20072039374192308No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA730.20072039374192308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.19522120487228134No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA690.1897220160026396No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT680.18697242156781874No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA660.181473232698177No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA620.17047485495889356No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA580.1594764772196101No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA540.14847809948032664No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA530.1457285050455058No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA500.1374797217410432No Hit
GTGAATGGGGTAGACGTAAAAAAAAAAAAAAAA480.13198053287140146No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA460.12648134400175975No Hit
ACGCAGAGTGAATGGGTAGACGTAAAAAAAAAA450.12373174956693887No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA450.12373174956693887No Hit
GCTAGACGTAGCTCACGTACTCTGCGTTGATAC410.11273337182765543No Hit
GCCTAGACGTAGCTCACGTACTCTGCGTTGATA400.10998377739283456No Hit
GTATAGACGTAGCTCACGTACTCTGCGTTGATA400.10998377739283456No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA400.10998377739283456No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT390.1072341829580137No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT390.1072341829580137No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA390.1072341829580137No Hit
GTGAATGGGTAGACGTAGCAAAAAAAAAAAAAA380.10448458852319283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAG200.00850290827.01
ATACCAC306.97249E-527.025
TACCACT306.97249E-527.026
TTTAAGC200.00850290827.02
ACCACTG351.9866423E-423.14285927
TGGGCTA350.00543241519.2857156
TGGGAGC552.0753767E-417.1818186
ATGGGCC500.002191729416.212
TATCAAC2300.014.0869561
ATGGGAT600.007367594613.57
ATGGGAG802.288924E-413.55
GATACCA600.007367594613.524
TGGGGAG600.007367594613.56
TTGATAC1051.1893022E-512.85714327
GTTGATA1759.513315E-1012.34285627
TCAACGC2800.011.5714293
ATCAACG2800.011.5714292
CAACGCA3000.010.7999994
CGTTGAT2950.010.52542427
AGAGTGA5300.09.6792455
Produced by FastQC (version 0.11.2)