FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_49.3520000005c335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_49.3520000005c335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55091
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA16843.0567606324081975No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA14772.6810186781869993No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA6811.236136574032056No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA6741.223430324372402No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA5961.0818463995934No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA5420.9838267593617831No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5320.9656749741337061No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA5250.952968724474052No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA4950.8985133687898205No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA4380.7950481929897805No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA4070.7387776587827413No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA3640.6607249823020094No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.6443883755967399No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA3530.6407580185511245No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA3080.5590749850247773No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT3010.5463687353651232No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA2870.5209562360458151No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA2850.5173258790001997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.5118803434317765No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA2610.47376159445281446No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA2600.47194641593000675No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC2560.46468570183877583No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.4610553447931604No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA2180.3957089179720825No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.39207856092646715No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT2020.3666660616071591No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT1980.3594053475159282No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1840.3339928481966202No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA1800.32673213410538926No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA1710.3103955274001198No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA1700.30858034887731206No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA1650.2995044562632735No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA1620.29405892069485035No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA1520.27590713546677315No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA1500.2722767784211577No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA1490.27046159989835No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA1440.26138570728431143No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA1440.26138570728431143No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA1400.2541249931930805No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA1400.2541249931930805No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA1370.2486794576246574No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA1310.2377883864878111No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC1270.2305276723965802No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA1240.22508213682815706No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.22326695830534932No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.21782142273692617No Hit
CACACGTACTCTGAAAAAAAAAAAAAAAAAAAA1170.212375887168503No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.2105607086456953No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA1110.2014848160316567No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA1100.19966963750884897No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG1090.19785445898604126No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA1010.18333303080357954No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCT980.1778874952351564No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.17607231671234866No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA970.17607231671234866No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA940.1706267811439255No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA940.1706267811439255No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA930.1688116026211178No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA930.1688116026211178No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA900.16336606705269463No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA900.16336606705269463No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA870.1579205314842715No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA860.15610535296146377No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC860.15610535296146377No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA840.15247499591584832No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA820.1488446388702329No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.14702946034742517No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.14158392477900203No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA770.13976874625619432No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA770.13976874625619432No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.13976874625619432No Hit
AACGTAGAGTGAATGGGCAGACACACGTACTCA750.13613838921057886No Hit
AACGCAGAGTGAATGGGCAGACACACGTAAAAA730.13250803216496343No Hit
CACATACTCAAAAAAAAAAAAAAAAAAAAAAAA710.128877675119348No Hit
GACACACGTACTCTAAAAAAAAAAAAAAAAAAA710.128877675119348No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA690.12524731807373254No Hit
GCAGACACACGTACTCAAAAAAAAAAAAAAAAA670.12161696102811712No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA660.1198017825053094No Hit
TCAACGCAGAGTGAATGGGCAGACACACGTACT650.11798660398250169No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG650.11798660398250169No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11617142545969396No Hit
GTGAATGGGCAGACACACGTACAAAAAAAAAAA630.11435624693688624No Hit
CGTACTCTAAAAAAAAAAAAAAAAAAAAAAAAA630.11435624693688624No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA610.1107258898912708No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGA610.1107258898912708No Hit
CACACGTACAAAAAAAAAAAAAAAAAAAAAAAA600.10891071136846309No Hit
ACGCTGAGTGAATGGGCAGACACACGTACTCAA600.10891071136846309No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTA600.10891071136846309No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA600.10891071136846309No Hit
GAGTGAATGGGCAGACACACGTAAAAAAAAAAA590.10709553284565537No Hit
ACACACGTACTCAAAAAAAAAAAAAAAAAAAAA590.10709553284565537No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA580.10528035432284764No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA570.10346517580003993No Hit
GTGAATGGGCAGACACACGTACTCTAAAAAAAA570.10346517580003993No Hit
AGACACACGTACTCAAAAAAAAAAAAAAAAAAA570.10346517580003993No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACAA570.10346517580003993No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.10164999727723223No Hit
TCAACGCAGAGTGAATGGGCAGACACAAAAAAA560.10164999727723223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG455.437323E-827.02
CTGAGTG455.437323E-827.04
ACGCTGA455.437323E-827.01
GAGTAAA200.00854059527.01
GCTGAGT455.437323E-827.03
GCACATG200.00854059527.05
TGAGTGA455.437323E-827.05
GTATCAT850.026.9999981
TATCATC850.026.9999982
AACGTAG501.5083424E-724.31
CAACTCA1100.023.318181
ATCATCG950.022.7368433
TCATCGC950.022.7368434
TCGCAGA1000.021.5999987
ATCGCAG1000.021.5999986
CATCGCA1050.020.5714285
GTAGAGT559.397441E-619.6363624
TAGAGTG559.397441E-619.6363625
GTATCTA350.005467599319.2857151
GGTATCA701.7776438E-719.2857151
Produced by FastQC (version 0.11.2)