FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_48.3520000005c31b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_48.3520000005c31b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43450
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA8281.905638665132336No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA1650.37974683544303794No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA1610.3705408515535098No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.35443037974683544No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA1260.2899884925201381No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA1200.2761795166858458No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA1080.24856156501726123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.20713463751438435No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA800.18411967779056385No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA770.17721518987341772No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA740.17031070195627157No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA700.1611047180667434No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT670.15420023014959722No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA670.15420023014959722No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC670.15420023014959722No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA660.1518987341772152No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA650.14959723820483314No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA630.14499424626006904No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA630.14499424626006904No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA620.14269275028768702No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA620.14269275028768702No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA560.1288837744533947No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA560.1288837744533947No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA550.12658227848101267No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA530.12197928653624857No Hit
GTACGTTAAGCGTGGAAAAAAAAAAAAAAAAAA520.11967779056386652No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA520.11967779056386652No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA520.11967779056386652No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA500.11507479861910241No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA490.11277330264672038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA257.7064056E-427.01
TGATACC1055.456968E-1219.28571326
TATCAAC2450.018.1836721
GATACCA1152.3646862E-1117.60869627
GTTGATA1204.5474735E-1116.87524
TTGATAC1204.5474735E-1116.87525
GCGTTGA3200.016.45312527
CGTTGAT1301.5279511E-1015.57692223
ATCAACG2850.014.684212
ATGGGGA852.3861741E-514.2941185
TCAACGC2850.014.21052553
CAACGCA3000.013.4999994
TCTGCGT9200.011.7391327
ATGGGTT2006.4210326E-1011.4755
TGCGTTG4650.011.3225826
TGGGTTC2059.677024E-1011.1951226
AATGGGT2700.011.04
AACGCAG3700.010.5810815
TGGGGTT1301.0662177E-410.3846156
CTGCGTT5300.09.93396325
Produced by FastQC (version 0.11.2)