FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_47.3520000005c2f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_47.3520000005c2f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80357
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2850.35466729718630613No Hit
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA2810.3496895105591299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.22151150490934204No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1520.1891558918326966No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC1340.16675585201040358No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1260.15680027875605113No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1240.154311385442463No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1210.15057804547208084No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC1160.14435581218811055No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.13813357890414027No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1110.13813357890414027No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1090.13564468559055218No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC960.11946687905222941No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA900.11200019911146508No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA830.10328907251390669No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA810.10080017920031858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACT257.747429E-427.00996820
TCATCGC751.8189894E-1225.1936054
CATCGCA751.8189894E-1225.1936055
GTATCAT701.0913936E-1125.0650671
TCGCAGA701.0913936E-1125.0650677
ATCGCAG701.0913936E-1125.0650676
ATCATCG801.8189894E-1223.6190093
TAACTTA300.002241628722.50830722
CAACTCA603.5483026E-822.4942911
TATCATC855.456968E-1222.2296542
TCAGAGT553.8388134E-722.0853045
ACTCAGA658.275674E-820.7639623
CTCAGAG608.863226E-720.2448644
TCATTCC404.9802277E-420.2448645
AACTTAC350.005477369819.29283323
GACGTGT350.005497152519.280826
CGTGTGC350.005497152519.280828
ACGTGTG350.005497152519.280827
TCAGACG501.02156875E-418.8952053
ATCTACG450.001099918317.9954342
Produced by FastQC (version 0.11.2)