FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_46.3520000005c2d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_46.3520000005c2d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122663
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.38642459421341396No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC4340.3538149238156575No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA4200.3424015391764428No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA3270.266584055501659No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1690.137775857430521No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1500.1222862639915867No Hit
CTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.11821005519186716No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.1002747364731011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC700.026.9995943
CTACGCA700.026.9995944
CGCTGAG307.10798E-526.9995922
CTGAGTG257.7884266E-426.9995924
CGCAGTG200.00858155226.9995927
CCTCAGC257.7884266E-426.9995923
GCTGAGT257.7884266E-426.9995923
GCATTCT200.00858155226.9995924
TGAGTGA257.7884266E-426.9995925
TGGCCCT257.7884266E-426.9995924
ATCTACG751.8189894E-1225.1996212
GTATCAT601.3096724E-924.7597241
TATCATC601.3151293E-924.7496262
TATCTAC801.8189894E-1223.6342831
TACGCAG752.910383E-1123.3996475
ACGCTGA352.0192226E-423.1519491
TCAGAGT653.3232936E-922.8458085
ACTCAGA603.578316E-822.499663
CTCAGAG603.578316E-822.499664
CGGCCCT300.002253374322.499664
Produced by FastQC (version 0.11.2)