FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_45.3520000005c2bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_45.3520000005c2bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47291
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1920.40599691273180943No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1140.24106066693451184No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1020.21568585988877378No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1000.21145672538115073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.20934215812733925No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA870.18396735108160114No Hit
GTGAATGGGTAGCGTCGAGATAAAAAAAAAAAA870.18396735108160114No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA840.1776236493201666No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT820.17339451481254362No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA800.1691653803049206No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA800.1691653803049206No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA780.1649362457972976No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA740.15647797678205155No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA680.1437905732591825No Hit
GTGAATGGGTAGCGTCGAGAAAAAAAAAAAAAA610.12898860248250196No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCGAGAT600.12687403522869045No Hit
GTGAATGGGATAGCGTCGAGAAAAAAAAAAAAA580.12264490072106743No Hit
GTGAATGGGGTATCTCGACGCTAAAAAAAAAAA560.11841576621344442No Hit
GTGAATGGGGTAGCGTAAAAAAAAAAAAAAAAA510.10784292994438689No Hit
TATCAACGCAGAGTGAATGGGATAGCGTCGAGA500.10572836269057537No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA490.10361379543676387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC3150.017.1428591
TGCGTTG4100.015.14634127
ATCAACG3450.014.8695652
TCAACGC3550.014.0704223
TCCTAGC600.0074108813.56
ACCTAGC904.1031533E-513.56
TCTGCGT11300.012.66371627
GTATCAA2350.012.6382981
CAACGCA3950.011.9620254
CTGCGTT5900.010.52542426
AACGCAG4750.010.2315795
AATGGGC1351.5685099E-410.04
CTCTGCG14750.09.701694526
GTTGATA1701.5345116E-59.52941127
TGGGATA1300.00124462789.3461546
ATGGGAT1608.3411185E-59.281255
ACTCTGC15500.09.23225925
TGGGGTA1350.00175620879.06
GGGATAG1200.0068363229.07
CCTAGCG3006.184564E-109.07
Produced by FastQC (version 0.11.2)