FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_43.3520000005c279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_43.3520000005c279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71351
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC7130.9992852237529958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.6208742694566299No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA3750.5255707698560637No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA2950.41344900562010345No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2450.34337290297262824No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA2300.32235007217838574No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT1820.25507701363680957No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA1580.2214404843660215No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA1370.19200852125408194No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA1370.19200852125408194No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1370.19200852125408194No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG1310.18359938893638492No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA1310.18359938893638492No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1220.17098569045983938No Hit
GTGAATGGGACATCCAGCTAAAAAAAAAAAAAA1200.1681826463539404No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1160.16257655814214236No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1120.15697046993034436No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA980.1373491611890513No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA970.1359476391361018No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA950.1331445950302028No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA930.1303415509243038No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA790.11072024218301076No Hit
CCCCCCACATCCAGCTAGACGTACTCTGCGTTG790.11072024218301076No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA780.10931872013006125No Hit
CCCCTACATCCAGCTAGACGTACTCTGCGTTGA780.10931872013006125No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA740.10371263191826323No Hit
AACGCAGAGTGAATGGGACATCCAGCAAAAAAA740.10371263191826323No Hit
ACGCAGAGTGAATGGGACATCCAGCTAGAAAAA730.10231110986531372No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT720.10090958781236424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT200.00855722127.04
ATGACAT200.00855722127.06
ACGTAGA200.00855722127.02
CGTAGAG200.00855722127.03
TGGGGTA200.00855722127.06
TAGAGTG200.00855722127.05
GTATCAT601.2914825E-924.751
GATACAT300.002243983622.56
ATCATCG603.5246558E-822.53
TCATCGC603.5246558E-822.54
CATCGCA603.5246558E-822.55
TATCATC707.677954E-921.2142872
TCGCAGA658.220559E-820.7692327
ATCGCAG658.220559E-820.7692326
AACACAT857.129165E-817.4705896
TGAATGA552.1111968E-417.1818182
GGGGAGT807.2961484E-716.8757
CAGCTAA604.099067E-415.7515
GAATGAG604.099067E-415.753
TATCAAC4950.015.2727271
Produced by FastQC (version 0.11.2)