FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_40.3520000005c21c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_40.3520000005c21c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45188
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA6171.3654067451535805No Hit
GTCAAGCTCAACACGTACTCTGCGTTGATACCA4330.9582189961936797No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA3190.7059396299902628No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2420.5355404089581305No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA1890.4182526334425069No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA1220.26998318137558647No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1200.265557227582544No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA1170.2589182968929804No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1170.2589182968929804No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1120.24785341241037442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.23900150482428964No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1060.23457555103124725No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA1050.23236257413472602No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA1050.23236257413472602No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA1010.2235106665486412No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA1000.22129768965212004No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA980.21687173585907762No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA970.21465875896255643No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA970.21465875896255643No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA950.21023280516951404No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA930.20580685137647162No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA890.19695494379038683No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA880.19474196689386564No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC830.18367708241125963No Hit
AGCTCAACACGTACTCAAAAAAAAAAAAAAAAA820.18146410551473843No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA770.17039922103213243No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC760.1681862441356112No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA730.1615473134460476No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC720.15933433654952642No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA700.15490838275648403No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA660.1460564751703992No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.13941754448083563No Hit
ACGCAGAGTGAATGGGTCAAGCTAAAAAAAAAA570.12613968310170842No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA560.12392670620518721No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC550.12171372930866603No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA520.11507479861910241No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAC520.11507479861910241No Hit
AACGCAGAGTGAATGGGTCAAGCTCAACACGTA510.1128618217225812No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA500.11064884482606002No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.10843586792953881No Hit
CTCAACACGTACTCAAAAAAAAAAAAAAAAAAA480.10622289103301762No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC470.1040099141364964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCTT200.00852629226.99889213
GTATCAT300.002217506322.5240211
ATGTCAA404.92677E-420.2491687
GATACCA703.7900809E-617.3564327
TATCAAC2550.015.3693321
TCTGCGT7250.013.03394827
CAGTCAA853.8564808E-412.7053627
ATCAACG3150.012.42806152
TGATACC1001.0996929E-412.14950226
TCAACGC3300.011.863153
TGCGTTG3250.010.79955827
CTCTGCG8850.010.67752826
CAACGCA3750.010.4395714
ACTCTGC9450.09.9995925
GCGTTGA2001.037246E-69.44961327
CTGCGTT3750.09.35961526
CGTCAAG5500.09.326898
TACTCTG10300.09.17438124
GTACTCT10400.09.08616523
GTACTCA2553.5121047E-88.99963110
Produced by FastQC (version 0.11.2)