FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_35.3520000005c16d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_35.3520000005c16d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97996
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC7960.8122780521653945No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.5908404424670395No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.4306298216253725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.4000163271970284No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2400.24490795542675212No Hit
CCCCCAGTCCTTCTGTGACGTACTCTGCGTTGA1440.14694477325605126No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1210.12347442752765418No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1120.114290379199151No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1110.11326992938487286No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA1060.10816768031348219No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA1030.10510633087064777No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1010.10306543124209151No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC1010.10306543124209151No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1010.10306543124209151No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1010.10306543124209151No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.1000040817992571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT257.743611E-427.01946626
CTTACTC257.743611E-427.01946625
TGTGACT257.766845E-427.0056320
TGACTTA257.766845E-427.0056322
ATTAACG257.766845E-427.005633
GTGACTT257.766845E-427.0056321
TTAACGC257.766845E-427.005634
TAACGCA257.790137E-426.991815
TATTAAC257.790137E-426.991812
GTATTAA257.8134856E-426.9780061
ACGCTGA406.0276216E-726.9780041
CAACTCA800.026.9780041
GGATCAG200.00860669726.9780041
ACCCCCT200.00860669726.9780041
TATCATC1450.025.1303062
ATCATCG1400.025.076663
TCATCGC1350.025.0052174
CATCGCA1350.024.992425
GTATCAT1450.024.1871781
TCGCAGA1400.024.0998337
Produced by FastQC (version 0.11.2)