FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_34.3520000005c144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_34.3520000005c144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65774
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA4330.6583148356493447No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2600.39529297290722776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.24325721409675555No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.22805363821570834No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA1400.2128500623346611No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1350.2052482743941375No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA1240.18852434092498555No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA1230.18700398333688084No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA1160.17636148022014778No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1100.16723933469151944No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1080.16419861951531No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA1030.15659683157478638No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA1030.15659683157478638No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT980.14899504363426277No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA960.14595432845805334No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA960.14595432845805334No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA940.14291361328184388No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA930.14139325569373917No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA920.13987289810563444No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA900.136832182929425No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA890.13531182534132027No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA880.13379146775321554No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA880.13379146775321554No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC850.12923039498890138No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA800.12162860704837777No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA800.12162860704837777No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA760.11554717669595889No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA750.11402681910785417No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA740.11250646151974944No Hit
GAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA730.11098610393164472No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA720.10946574634354No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC710.10794538875543527No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA700.10642503116733056No Hit
GTGAATGGGATCGAGACGAGTAAAAAAAAAAAA680.1033843159911211No Hit
AGTACGTACAAAAAAAAAAAAAAAAAAAAAAAA680.1033843159911211No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10186395840301639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACAG200.0085240827.0183092
GTATCTA257.718247E-427.0183071
GTATCAT554.656613E-1027.0183071
CAACTCA604.3655746E-1127.0183051
ACACAGA200.00855588626.9977133
TGTATAC200.00855588626.9977133
CACAGAG200.00855588626.9977134
AACTCAG651.1823431E-1024.9399762
TATCATC702.9831426E-1023.1585482
TCATCGC603.517016E-822.4980954
CATCGCA603.517016E-822.4980955
TCGCAGA603.545574E-822.4809597
ATCGCAG603.545574E-822.4809596
ACTCAGA757.1486284E-1021.5981713
CTCAGAG757.1486284E-1021.5981714
CGCTGAG404.9356866E-420.2637312
ACGCTGA404.9356866E-420.2637311
CTGAGTG404.961185E-420.2482854
TCAGAGT801.6007107E-920.2482855
GCTGAGT404.961185E-420.2482853
Produced by FastQC (version 0.11.2)