FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_33.3520000005c12a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_33.3520000005c12a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41951
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2630.6269218850563754No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1740.4147696121665753No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC1720.4100021453600629No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1590.3790136111177326No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1340.3194202760363281No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1330.3170365426330719No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC1310.31226907582655955No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA1240.29558294200376634No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA1230.29319920860051013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.2884317417939978No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1210.2884317417939978No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1180.2812805415842292No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1130.2693618745679483No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC1050.2502920073418989No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA990.2359896069223618No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA970.23122214011584946No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT920.21930347309956855No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA900.2145360062930562No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA770.18354747205072583No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA730.17401253843770112No Hit
TAGCCATTCCGTTACGTACTCTGCGTTGATACC720.17162880503444494No Hit
ACGCAGAGTACGTAAAAAAAAAAAAAAAAAAAA610.14540773759862696No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.14540773759862696No Hit
AAGCCATTCCGTTACGTACTCTGCGTTGATACC590.1406402707921146No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA570.13587280398560225No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA560.13348907058234608No Hit
GTGAATGGGGAGTACGTAACGGAAAAAAAAAAA490.11680293675955282No Hit
GAGTACGTAAAAAAAAAAAAAAAAAAAAAAAAA490.11680293675955282No Hit
GTGAATGGGGAGCCAAAAAAAAAAAAAAAAAAA490.11680293675955282No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT460.10965173654978427No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTAACG460.10965173654978427No Hit
GTGAATGGGGTACGTAACGAAAAAAAAAAAAAA450.1072680031465281No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10250053634001573No Hit
GAGTGAATGGGGAGTACGTAACGAAAAAAAAAA430.10250053634001573No Hit
GTGAATGGGGTACGTAACGGAAAAAAAAAAAAA430.10250053634001573No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA420.10011680293675956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG300.002232145322.494045
GCGTTGA3600.018.76740327
TATCAAC2300.012.3228231
TGCGTTG6450.012.15080327
TGATACC906.175221E-412.01113827
AATGGGT800.003559414811.8093714
TTGATAC850.00554704911.12796626
GTTGATA900.00848395810.50974625
ATCAACG2655.638867E-1110.1859812
CAAGCCA1607.424438E-610.1223187
ATGGGAG1751.95153E-610.0259155
TAAGCCA1100.00332993089.8155817
CTGCGTT8000.09.79658426
TCTGCGT12300.09.55764327
TCAACGC2852.2919266E-109.4711743
AAGCCAT3501.8189894E-129.2546918
CAGAGTG5800.08.6097884
AGAGTGA5800.08.6097885
ACGCAGA6500.08.5131291
CGCAGAG6500.08.5131292
Produced by FastQC (version 0.11.2)