FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_30.3520000005c0d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_30.3520000005c0d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64273
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA4180.650350847167551No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2740.4263065361816003No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA2370.36873959516437693No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA2170.3376223297496616No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA1900.29561402143979587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.267608482566552No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1670.2598291662128732No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1610.2504939865884586No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1560.24271467023477977No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1530.23804708042257247No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA1510.23493535388110096No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1430.2224884477152148No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1310.20381808846638558No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA1290.20070636192491406No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1290.20070636192491406No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1290.20070636192491406No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA1220.18981531902976367No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA1180.1835918659468206No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA1130.17581254959314177No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA910.14158355763695488No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA890.13847183109548333No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC870.1353601045540118No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA860.133804241283276No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA830.12913665147106873No Hit
ACGCAGAGTGAATGGGTTCGTCACAAAAAAAAA830.12913665147106873No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA790.12291319838812564No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA770.11980147184665411No Hit
GTGAATGGGGTTCGTCACACTACGTACTCAAAA760.11824560857591836No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.11668974530518257No Hit
GTGAATGGGGTTCGTCACAAAAAAAAAAAAAAA720.11202215549297528No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA690.10735456568076798No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA680.1057987024100322No Hit
AACGCAGAGTGAATGGGTTCGTCACACTACGTA660.10268697586856067No Hit
GTGAATGGGTTCGTCACACTACGTACAAAAAAA660.10268697586856067No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA650.10113111259782491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA405.918737E-727.0062451
ACTCCCC200.00854122827.0062451
TACGCAG257.771877E-426.9851825
GTATCAT553.7959035E-722.0960181
ATCATCG553.7959035E-722.0960183
TCATCGC553.7959035E-722.0960184
TCGCAGA553.8246435E-722.0787837
ATCGCAG553.8246435E-722.0787836
AGGTTCG454.5353467E-520.9884727
TATCATC608.764746E-720.2546832
CATCGCA608.8308843E-720.2388845
CGCTGAG350.005469882419.2901742
CTCAGAG501.01367696E-418.9043734
AACTCAG552.1032694E-417.1857932
ACTCAGA552.1032694E-417.1857933
TCTGCGT12000.016.54132527
ACGTTCG1750.014.64909657
TCAGAGT657.543958E-414.5304815
GCGTTGA2700.014.50335427
GTGTTCG954.3768996E-614.2027257
Produced by FastQC (version 0.11.2)