FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_29.3520000005c0be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_29.3520000005c0be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104661
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA12351.1800001910931484No Hit
TGCTTGGACCTTACGTACTCTGCGTTGATACCA11201.070121630788928No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC9410.8990932630110547No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC5550.5302834866855849No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA5310.507352308882965No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA4820.4605344875359494No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA4810.45957902179417354No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA4210.4022510772876239No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA4040.3860081596774348No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA3430.32772474942910923No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA3310.3162591605277993No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA3310.3162591605277993No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA3260.31148183181892014No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA3050.2914170512416277No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA2850.27230773640611117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.2713522706643353No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT2740.261797613246577No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT2740.261797613246577No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA2590.24746562711993964No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2460.23504457247685387No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA2360.22548991505909555No Hit
ACGCAGAGTGAATGGGACCTTACGTACTCAAAA2260.21593525764133725No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA2130.2035142029982515No Hit
GGACCTTACGTACTCAAAAAAAAAAAAAAAAAA2080.19873687428937237No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA2060.1968259428058207No Hit
GATACCACTGCTTGGACCTTACGTACTCTGCGT2000.19109314835516572No Hit
GCCTTACGTACTCTGCGTAAAAAAAAAAAAAAA1800.17198383351964913No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT1800.17198383351964913No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA1720.1643401075854425No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.15860731313478757No Hit
TACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA1520.14523079274992595No Hit
GTATCATCGCAGAGTGAATGGGCCTTACGTACT1510.14427532700815013No Hit
GAGTGAATGGGACCTTACGTACTCAAAAAAAAA1510.14427532700815013No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.13949799829927098No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA1340.12803240939796104No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.12707694365618522No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA1310.12516601217263357No Hit
GTATCAACGCAGAGTGAATGGGACCTTACGTAC1280.12229961494730607No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA1240.11847775198020274No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.1165668204966511No Hit
CCTTACGTACTCTGCGAAAAAAAAAAAAAAAAA1200.11465588901309944No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA1180.11274495752954777No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCTGCG1180.11274495752954777No Hit
TACGTACTCTGCGTTGATACCAAAAAAAAAAAA1160.11083402604599613No Hit
ATCAACGCAGAGTGAATGGGCCTTACGTACTCA1150.10987856030422029No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA1140.10892309456244446No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1130.10796762882066863No Hit
GCCTTACGTACTCTGCGAAAAAAAAAAAAAAAA1110.10605669733711696No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA1110.10605669733711696No Hit
AACGCAGAGTGAATGGGACCTTACGTACTCAAA1070.10223483437001367No Hit
TATCTACGCAGAGTGAATGGGCCTTACGTACTC1050.10032390288646201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTCA200.00855898627.0105171
CCACGTG200.00857897726.9976124
GTATCAT1050.024.4380871
TATCATC1050.024.426412
CAACTCA352.0165158E-423.151871
ACTCAGA352.0230642E-423.140813
CTCAGAG352.0230642E-423.140814
TCAGAGT300.002252143622.498015
TGAGTGA1000.021.598095
AACTCAG454.5546152E-520.9981422
ACGCTGA1050.020.5794411
CTGAGTG1050.020.5696094
TCGCAGA1250.020.5181857
ATCATCG1250.020.5181853
ATCGCAG1250.020.5181856
TCATCGC1250.020.5181854
AGTAAAT404.9885956E-420.2482092
TATCTAC1100.019.644011
ATCTACG1100.019.6346262
TCTACGC1100.019.6346263
Produced by FastQC (version 0.11.2)