FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_28.3520000005c095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_28.3520000005c095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60251
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA5400.8962506846359396No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT3080.5111948349404989No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA2140.3551808268742428No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2100.34854193291397656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.2539376939801829No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1270.2107848832384525No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1210.20082654229805316No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1190.19750709531792002No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA1130.1875487543775207No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1120.18588903088745415No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1090.1809098604172545No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA1020.16929179598678862No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA980.16265290202652238No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA970.16099317853645584No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA950.15767373155632272No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC920.15269456108612306No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA900.14937511410598994No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA810.13443760269539096No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT740.12281953826492507No Hit
ACGCAGAGTGAATGGGATCTCGTCCTCAAAAAA730.1211598147748585No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA710.11784036779472541No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11618064430465884No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA670.11120147383445918No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC640.10622230336425952No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA640.10622230336425952No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1029028563841264No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA610.10124313289405985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT604.3655746E-1127.0016631
GTAATCT200.00854422627.0016613
TATCATC651.1823431E-1024.9246122
CACAATC300.002239139522.5013875
TGGGAGA300.00224999922.4826976
ATCATCG658.1616236E-820.770513
TCATCGC658.1616236E-820.770514
CATCGCA658.1616236E-820.770515
TCGCAGA658.2338374E-820.753267
ATCGCAG658.2338374E-820.753266
TACCACT450.001087135618.01608527
ACAATCT703.85973E-617.3437966
TATCAAC4050.016.0009861
GCGTTGA4600.015.86198727
CAGAGAG604.0838806E-415.75097110
GCAGAGA604.0838806E-415.7509719
GACATCT550.004200735614.7159476
AACATCT751.355236E-414.3889266
ATACCAC600.00743926313.50083226
ATCAACG4900.013.5008322
Produced by FastQC (version 0.11.2)