FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_27.3520000005c07b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_27.3520000005c07b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52090
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2010.38587060856210403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.29372240353234785No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA1150.2207717412171242No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1090.20925321558840468No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA1050.2015741985025917No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT990.19005567287387212No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA880.16893837588788635No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA860.16509886734497986No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA830.15933960453062007No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA830.15933960453062007No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA820.15741985025916683No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA800.1535803417162603No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA760.1459013246304473No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA700.13438279900172778No Hit
GTGAATGGGGTCGTTGGAAAAAAAAAAAAAAAA670.12862353618736802No Hit
GGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.12670378191591475No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA600.11518525628719525No Hit
GTGAATGGGGTACGTTTCGACCAAAAAAAAAAA600.11518525628719525No Hit
GTACGTTTCGACCAAAAAAAAAAAAAAAAAAAA570.10942599347283548No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA540.10366673065847572No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA530.10174697638702247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG200.00853638727.02
CTGAGTG200.00853638727.04
ACGCTGA200.00853638727.01
GCTGAGT200.00853638727.03
AACTCAG451.6463491E-624.02
TCAGAGT451.6463491E-624.05
GTATCAT352.0028971E-423.1428591
CAACTCA504.1117855E-621.5999981
ACTCAGA504.1117855E-621.5999983
CTCAGAG504.1117855E-621.5999984
TATCTAC559.381236E-619.6363621
GAGTAAA350.005463666319.2857151
ATCTACG501.0108189E-418.92
TACGCAG601.9863564E-518.05
TCTACGC552.0974169E-417.1818183
CTACGCA552.0974169E-417.1818184
TGCGTTG3750.016.5627
TATCATC500.002209065516.1999992
TCGCAGA500.002209065516.1999997
ATCGCAG500.002209065516.1999996
Produced by FastQC (version 0.11.2)