FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_25.3520000005c02b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_25.3520000005c02b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18784
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1170.6228705281090289No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA1170.6228705281090289No Hit
CAACGAACCAAGACGTACTCTGCGTTGATACCA860.4578364565587734No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA850.45251277683134583No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA730.38862862010221466No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA640.34071550255536626No Hit
GTGAATGGGACAACGAACCAAAAAAAAAAAAAA640.34071550255536626No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA620.3300681431005111No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA610.3247444633730835No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA600.31942078364565585No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA540.2874787052810903No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA510.2715076660988075No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAG510.2715076660988075No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT490.26086030664395227No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA490.26086030664395227No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA470.25021294718909715No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAA460.24488926746166953No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA450.2395655877342419No Hit
AACCAAGACGAAAAAAAAAAAAAAAAAAAAAAA430.2289182282793867No Hit
TATCAACGCAGAGTGAATGGGACAACGAACCAA410.2182708688245315No Hit
GTATCAACGCAGAGTGAATGGGCAACGAACCAA400.21294718909710392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.2076235093696763No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA380.2022998296422487No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA380.2022998296422487No Hit
GTGAATGGGGCAACGAACCAAAAAAAAAAAAAA360.19165247018739354No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACGTA350.18632879045996592No Hit
CAACGAACCAAAAAAAAAAAAAAAAAAAAAAAA350.18632879045996592No Hit
GAGTGAATGGGACAACGAACCAAAAAAAAAAAA340.18100511073253833No Hit
AACGCAGAGTGAATGGGCAACGAACCAAAAAAA330.17568143100511074No Hit
TATCAACGCAGAGTGAATGGGGCAACGAACCAA320.17035775127768313No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA310.16503407155025554No Hit
GTGAATGGGACAACGAACCAAGACAAAAAAAAA300.15971039182282792No Hit
GAGTGAATGGGCAACGAACAAAAAAAAAAAAAA290.15438671209540034No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA290.15438671209540034No Hit
TATCAACGCAGAGTGAATGGGCAACGAACAAAA290.15438671209540034No Hit
GTGAATGGGCCAACGAACCAAAAAAAAAAAAAA280.14906303236797275No Hit
AACCAAGACGTAAAAAAAAAAAAAAAAAAAAAA270.14373935264054516No Hit
GAGTGAATGGGCAACGAACCAAAAAAAAAAAAA270.14373935264054516No Hit
GTGAATGGGACAACGAACAAAAAAAAAAAAAAA260.13841567291311754No Hit
GTATCAACGCAGAGTGAATGGGACAACGAACCA260.13841567291311754No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA260.13841567291311754No Hit
ACGCAGAGTGAATGGGCCAACGAACCAAAAAAA260.13841567291311754No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA250.13309199318568996No Hit
AACCAAGACGTACAAAAAAAAAAAAAAAAAAAA250.13309199318568996No Hit
AACGCAGAGTGAATGGGCAACGAACAAAAAAAA250.13309199318568996No Hit
GAATGGGACAACGAACCAAAAAAAAAAAAAAAA240.12776831345826234No Hit
GTCTTGGTTCGTTGAAAAAAAAAAAAAAAAAAA240.12776831345826234No Hit
ACCAAGACGTACTCAAAAAAAAAAAAAAAAAAA230.12244463373083476No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA230.12244463373083476No Hit
GTGAATGGGCCAACGAAAAAAAAAAAAAAAAAA220.11712095400340716No Hit
ACGCAGAGTGAATGGGACAACGAACCAAAAAAA220.11712095400340716No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA210.11179727427597956No Hit
GTGAATGGGCAACGAACCAAGACAAAAAAAAAA210.11179727427597956No Hit
CAACGAACCAAGACAAAAAAAAAAAAAAAAAAA210.11179727427597956No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11179727427597956No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACAAA210.11179727427597956No Hit
ATCAACGCAGAGTGAATGGGCAACGAACCAAGA200.10647359454855196No Hit
GCAACGAACCAAAAAAAAAAAAAAAAAAAAAAA200.10647359454855196No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC200.10647359454855196No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA200.10647359454855196No Hit
TATCAACGCAGAGTGAATGGGCCAACGAACCAA190.10114991482112436No Hit
GAGTGAATGGGCAACGAACCAAGACAAAAAAAA190.10114991482112436No Hit
GTGAATGGGGCAACGAAAAAAAAAAAAAAAAAA190.10114991482112436No Hit
GCAACGAACCAAGACGTACTCTGCGTTGATACC190.10114991482112436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1900.017.7631591
TCAACGC1950.017.3076933
ATGGGAG500.00213874716.25
TCTGCGT2500.015.65999927
ATGGGAC1352.2919266E-1015.05
CAACGCA2250.014.9999994
ATCAACG2400.014.06252
AATGGGA2950.013.7288134
TGGGACA1001.0343059E-412.1500016
AACGCAG2950.011.4406785
CTCTGCG3450.011.34782626
GTGAATG7600.010.1251
TGAATGG7600.010.1252
ACTCTGC3900.010.03846225
GAATGGG8050.09.7267083
TACTCTG4050.09.33333324
GTACTCT4350.09.023
CGCAGAG4250.08.576477
ACGCAGA4300.08.4767456
CAGAGTG4200.08.3571439
Produced by FastQC (version 0.11.2)