FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_21.3520000005bfb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_21.3520000005bfb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81679
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.30607622522312955No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2320.2840387370070642No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA2150.26322555369189143No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA2030.24853389488118122No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA1810.22159918706154583No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA1670.20445891844905056No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA1560.19099156453923286No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA1470.17997282043120016No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC1280.15671102731424233No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA1250.15303811261156477No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1250.15303811261156477No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1120.13712214889996205No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA1100.134673539098177No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC1090.1334492341972845No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1040.12732770969282192No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1020.12487909989103685No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA1000.12243049008925182No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.11753327048568175No Hit
ACGCAGAGTGAATGGGTACGTACTCTGCGTTGA900.11018744108032665No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT880.10773883127854161No Hit
GTATCAACGCAGAGTGAATGGGTACGTACTCTG860.10529022147675658No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA830.10161730677407901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGA200.00856338327.0006142
CGTAGAG200.00856338327.0006143
AGTAAAT200.00856338327.0006142
TATCATC200.00856338327.0006142
CGAGCCC200.00856338327.0006143
CATACCA257.764171E-427.0006145
CCGACCC200.00856338327.0006144
GTATCAT200.00858906726.984061
GAGTAAA257.792082E-426.9840581
CAACTCA307.107068E-526.9840561
ACGCTGA401.8347866E-523.6110521
TATCTAC401.8347866E-523.6110521
CGCTGAG352.0162009E-423.1433812
CTGAGTG352.0162009E-423.1433814
ATCTACG352.0162009E-423.1433812
GCTGAGT352.0162009E-423.1433813
CTACGCA352.0162009E-423.1433814
AGGCCCA300.002246444822.500515
CGACCCA300.002246444822.500515
CTCAACC300.002246444822.500514
Produced by FastQC (version 0.11.2)