FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_20.3520000005bf99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_20.3520000005bf99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84320
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT8300.9843453510436433No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA8280.9819734345351043No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA5490.6510910815939279No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA4560.540796963946869No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA2920.3462998102466793No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA2710.3213946869070209No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA2700.3202087286527514No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA2330.27632827324478176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.25379506641366223No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.2526091081593928No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA1800.21347248576850095No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA1720.20398481973434535No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA1610.1909392789373814No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC1580.18738140417457305No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.1838235294117647No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1460.17314990512333964No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.16603415559772297No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA1340.15891840607210628No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA1320.15654648956356737No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA1190.1411290322580645No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA1110.1316413662239089No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA1090.12926944971537002No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA1080.12808349146110057No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA1070.12689753320683111No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA1040.12333965844402277No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA1010.11978178368121442No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.10910815939278937No Hit
ACGTAGGAGACTAAAAAAAAAAAAAAAAAAAAA920.10910815939278937No Hit
CAACTCAGAGTGAATGGGACGTAGGAGACAAAA850.10080645161290322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG455.511174E-826.9994092
CTGAGTG455.511174E-826.9994094
ACGCTGA455.511174E-826.9994091
GTATCAT1300.026.9994091
GCTGAGT455.511174E-826.9994093
TTAACGC307.082305E-526.9994074
CCACCAC200.00856683826.9994054
TGAGTGA505.1022653E-926.9994055
CAACTCA1050.025.713721
AACTCAG1050.024.4280342
CTCAGAG1050.024.4280344
TATCATC1450.024.2063662
TCATCGC1450.024.2063664
TCAGAGT950.024.1573665
ATCATCG1500.023.3994853
ATCGCAG1500.023.3994856
ACTCAGA1100.023.3176693
TAACGCA352.0175855E-423.1423475
TCGCAGA1550.022.6446657
CATCGCA1600.021.9370175
Produced by FastQC (version 0.11.2)