FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_15.3520000005bef7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_15.3520000005bef7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53291
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC4470.8387907901897131No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2980.5591938601264753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.43159257660768235No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1600.30023831416186597No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1510.283349908990261No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1430.26833799328216773No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1410.2645850143551444No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1360.2552025670375861No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1350.2533260775740744No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA1340.2514495881105627No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1230.23080820401193447No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1160.21767277776735283No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA1000.18764894635116622No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC980.1838959674241429No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT970.18201947796063125No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA930.17451352010658458No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA810.15199564654444464No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.14824266761742133No Hit
GCTATGCCAACGTACTCAAAAAAAAAAAAAAAA730.13698373083635135No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA700.13135426244581636No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA690.1294777729823047No Hit
ATGCCAACGTACTCAAAAAAAAAAAAAAAAAAA660.12384830459176972No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT650.12197181512825805No Hit
GTGAATGGGTGTGAGCAACTCTAACTATGCCAA640.1200953256647464No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA640.1200953256647464No Hit
GCTATGCCAAAAAAAAAAAAAAAAAAAAAAAAA580.10883638888367642No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT580.10883638888367642No Hit
GTGAATGGGTCAGCTAAAAAAAAAAAAAAAAAA580.10883638888367642No Hit
CCCATCAGCTATGCCAACGTACTCTGCGTTGAT560.10508340995665308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG200.00853798527.06
ACGCTGA307.03294E-527.01
GTATCAT200.00853798527.01
TCTACGC257.7294395E-427.03
TACGCAG257.7294395E-427.05
TATCATC200.00853798527.02
CTACGCA257.7294395E-427.04
TACTCTT307.03294E-527.020
TGAGTGA307.03294E-527.05
TTCGTTG352.0036745E-423.14285925
ATCTACG352.0036745E-423.1428592
CTCTTCG352.0036745E-423.14285922
ACTCTTC352.0036745E-423.14285921
TCGTTGA352.0036745E-423.14285926
CTTCGTT352.0036745E-423.14285924
TCTTCGT352.0036745E-423.14285923
TATCTAC300.002236528322.51
GCTTCAG300.002236528322.56
TTCTCAG608.726347E-720.256
AGTGAAA404.9412943E-420.252
Produced by FastQC (version 0.11.2)