FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_13.3520000005bea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_13.3520000005bea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97762
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA5430.5554305353818457No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC4410.4510955176858084No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC3800.38869908553425664No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA3120.3191424070702318No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC2820.28845563715963257No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA2650.27106646754362634No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA2470.2526544055972668No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC2260.2311736666598474No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA2240.22912788199914075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.15752541887440927No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC1520.15547963421370267No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA1450.1483193879012295No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.14115914158875636No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.13911335692804974No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA1250.12786154129416336No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1100.11251815633886376No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA1100.11251815633886376No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA1030.10535791002639061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGG200.00856379727.0056343
GTATCAT455.5179044E-827.0056341
GTGTATG257.766845E-427.005631
TCGCAGA406.0006823E-726.9918127
TGGGTCA200.00858522626.9918126
ATCGCAG406.0006823E-726.9918126
CTACGCA551.4102625E-824.5505754
TACGCAG551.4179022E-824.5380125
ACGCTGA451.6682479E-624.0050091
CAACTCA451.6682479E-624.0050091
ATCATCG451.6682479E-624.0050093
CTGAGTG401.8282764E-523.6299294
CTATACC352.0176495E-423.1476883
TATACCC300.00224718922.5046924
GTATTAA300.00224718922.5046921
TCTACGC603.5508492E-822.5046923
TATCTAC603.5508492E-822.5046921
TCATCGC504.1659696E-621.6045064
CATCGCA504.184534E-621.5934495
CGCTGAG454.5401932E-521.0043832
Produced by FastQC (version 0.11.2)