FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate2_10.3520000005be57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate2_10.3520000005be57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56952
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA7141.2536873156342183No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA7021.2326169405815424No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA4930.8656412417474365No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA4690.8235004916420846No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA3130.5495856159572974No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA2600.45652479280797864No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT2530.4442337406939177No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA2340.41087231352718073No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA2200.38629020929905883No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA2000.35117291754459895No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA1970.34590532378142996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.319567354965585No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA1450.25460036521983426No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA1420.24933277145666524No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA1250.21948307346537438No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA1250.21948307346537438No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT1080.18963337547408343No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA990.1738305941845765No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA950.1668071358336845No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT900.15802781289506954No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA880.15451608371962353No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA850.14924848995645457No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.14573676078100856No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA820.14398089619328558No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA790.1387133024301166No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC770.1352015732546706No Hit
GACGTACGTACTCTAAAAAAAAAAAAAAAAAAA750.13168984407922463No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA720.12642225031605564No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA700.12291052114060963No Hit
GGAGAGACGTACGTACTCAAAAAAAAAAAAAAA670.11764292737744064No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA660.11588706278971765No Hit
ACGCAGAGTGAATGGGATGCAGAGACAAAAAAA660.11588706278971765No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA650.11413119820199466No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11237533361427167No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA640.11237533361427167No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA630.11061946902654868No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.11061946902654868No Hit
AGACGTACGTACTCAAAAAAAAAAAAAAAAAAA620.10886360443882567No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10535187526337969No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGA590.1035960106756567No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.10184014608793371No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG570.1000842815002107No Hit
ACGCAGAGTGAATGGGAGAGACGTACGTACTCA570.1000842815002107No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA570.1000842815002107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA200.00853755127.0035151
GTATCAT554.638423E-1027.0035151
TATCATC604.3655746E-1127.0035152
CATCCAT200.00857426826.9798075
ATCATCG601.2751116E-924.7532223
TCATCGC601.2751116E-924.7532224
TCGCAGA601.2896635E-924.7314917
ATCGCAG601.2896635E-924.7314916
TACCACT451.6363974E-624.02423527
CATCGCA653.255991E-922.8290675
CGCAAAG300.002236767422.5029282
CAACTCA855.456968E-1222.238191
AACTCAG953.092282E-1119.8973272
ACTCAGA953.092282E-1119.8973273
CTCAGAG953.092282E-1119.8973274
TCAGAGT953.092282E-1119.8798585
ATACCAC559.324704E-619.65619326
TATCAAC3400.016.2815321
GTATCAA2100.014.7876391
CCCCCAT550.00417194114.729191
Produced by FastQC (version 0.11.2)