FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_88.3520000005bc08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_88.3520000005bc08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13142
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2341.7805509054938367No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA1270.9663673717851164No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA880.6696088875361437No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA810.616344544209405No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA740.5630802008826662No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA630.4793790899406483No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA550.41850555471008977No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA520.39567797899863033No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA500.3804595951909907No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA500.3804595951909907No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA490.3728504032871709No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA460.35002282757571146No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC380.28914929234515296No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA370.2815401004413331No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA370.2815401004413331No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA360.27393090853751334No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC350.2663217166336935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.2663217166336935No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA340.2587125247298737No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT330.25110333282605385No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA330.25110333282605385No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA290.22066656521077463No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA290.22066656521077463No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA270.205448181403135No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA250.19022979759549535No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC250.19022979759549535No Hit
ACGCAGAGTGAATGGGTCAAGCAAAAAAAAAAA240.18262060569167554No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA220.16740222188403592No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.16740222188403592No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA200.1521838380763963No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA200.1521838380763963No Hit
ATCAACGCAGAGTGAATGGGTCAAGCTCAAAAA200.1521838380763963No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA200.1521838380763963No Hit
ACGCAGAGTGAATGGGTCAAGCTAAAAAAAAAA190.14457464617257648No Hit
GAGTGAATGGGGTCAAGCAAAAAAAAAAAAAAA190.14457464617257648No Hit
AGTCAAGCTCAACACGTACTCTGCGTTGATACC190.14457464617257648No Hit
ACGCAGAGTGAATGGGGTCAAGCAAAAAAAAAA180.13696545426875667No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA180.13696545426875667No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA170.12935626236493686No Hit
GAGTGAATGGGTCAAGCAAAAAAAAAAAAAAAA160.12174707046111702No Hit
GAGTGAATGGGGTCAAGCTCAAAAAAAAAAAAA160.12174707046111702No Hit
GAGCAAGACTGCATCTCAAAAAAAAAAAAAAAA160.12174707046111702No Hit
TATCAACGCAGAGTGAATGGGTCAAGCAAAAAA160.12174707046111702No Hit
GTGAATGGGGTCAAGCAAAAAAAAAAAAAAAAA160.12174707046111702No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA150.1141378785572972No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT140.1065286866534774No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA140.1065286866534774No Hit
AACGCAGAGTGAATGGGTCAAGCAAAAAAAAAA140.1065286866534774No Hit
ACGCAGAGTGAATGGGGTCAAGCTAAAAAAAAA140.1065286866534774No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1065286866534774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC901.13423084E-716.5000021
ATCAACG1003.762707E-714.852
TCAACGC1056.5407585E-714.1428573
CAACGCA1151.825767E-612.9130444
CAGAGTG2355.5661076E-1010.3404264
AGAGTGA2408.021743E-1010.1255
GCAGAGT2451.1496013E-99.9183673
CGCAGAG2451.1496013E-99.9183672
ACGCAGA2552.3028406E-99.5294111
AACGCAG1607.1662114E-59.281255
AGTGAAT2902.1093001E-88.3793117
GAGTGAA2952.8247086E-88.2372886
AATGGGT2008.711808E-58.14
ATGGGTC1550.0055455067.83870945
TGGGTCA1550.0055455067.83870946
GTGAATG3752.0645166E-76.841
TGAATGG3752.0645166E-76.842
GAATGGG4251.8194532E-66.0352943
Produced by FastQC (version 0.11.2)