FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_87.3520000005bbfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_87.3520000005bbfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20944
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA1410.6732238349885409No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1090.5204354469060352No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1020.487012987012987No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA880.42016806722689076No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA690.329449961802903No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA680.3246753246753247No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC660.31512605042016806No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA630.30080213903743314No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA600.28647822765469827No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA570.27215431627196335No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT530.2530557677616501No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA510.2435064935064935No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA510.2435064935064935No Hit
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA490.2339572192513369No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA450.2148586707410237No Hit
GTGAATGGGAGCTGACAAAAAAAAAAAAAAAAA420.2005347593582888No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT420.2005347593582888No Hit
GTGAATGGGAGCTGACGAAGAAAAAAAAAAAAA400.19098548510313218No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA400.19098548510313218No Hit
AACGCAGAGTGAATGGGAGCTGACGAAAAAAAA380.18143621084797554No Hit
GAGTGAATGGGGCTGACGAAAAAAAAAAAAAAA360.17188693659281895No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA360.17188693659281895No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA350.16711229946524064Illumina Single End PCR Primer 1 (100% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.16233766233766234No Hit
ACGCAGAGTGAATGGGGCTGACGAAAAAAAAAA340.16233766233766234No Hit
GAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA340.16233766233766234No Hit
AACGCAGAGTGAATGGGGCTGACGAAAAAAAAA310.14801375095492741No Hit
TATCAACGCAGAGTGAATGGGGCTGACGAAAAA290.13846447669977083No Hit
ACGCAGAGTGAATGGGCTGACGAAAAAAAAAAA280.1336898395721925No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAGT280.1336898395721925No Hit
CAGCTGACGAAGTACGTACTCTGCGTTGATACC280.1336898395721925No Hit
GAATGGGAGCTGACGAAAAAAAAAAAAAAAAAA270.1289152024446142No Hit
GTGAATGGGAGCTGACGAAGTACAAAAAAAAAA260.1241405653170359No Hit
GAATGGGGCTGACGAAAAAAAAAAAAAAAAAAA260.1241405653170359No Hit
GACGAAGTACGTACTCAAAAAAAAAAAAAAAAA260.1241405653170359No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA240.1145912910618793No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA230.109816653934301No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA220.10504201680672269No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10504201680672269No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10504201680672269No Hit
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA210.1002673796791444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG1301.3460522E-1015.57692227
TATCAAC1603.0358933E-912.656251
TCTGCGT4550.012.16483427
CTGCGTT2400.011.812527
TGGGAGC1153.8333752E-410.5652176
ATCAACG1701.2221626E-610.3235282
ATGGGAG1606.8384743E-610.1255
TCAACGC1751.7738075E-610.0285723
GTATCAA1659.868876E-69.8181821
CTCTGCG5700.09.47368426
AAGCTGA1651.0854696E-49.08
ACTCTGC6150.08.78048825
TACTCTG6150.08.78048824
GTACTCT6350.08.50393723
CAACGCA2101.8038045E-58.3571424
GCAGAGT3457.530616E-108.2173923
AAGTACG6750.08.217
AGTACGT6950.08.15827418
GGGAGCT1500.0043468158.0999997
CAGAGTG3703.3651304E-108.0270274
Produced by FastQC (version 0.11.2)