FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_86.3520000005bbd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_86.3520000005bbd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18964
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT2571.355199325036912No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2181.1495465091752795No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA1931.0177177810588485No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA1931.0177177810588485No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA1760.9280742459396751No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA1640.8647964564437883No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC1320.6960556844547563No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC1290.6802362370807846No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA1290.6802362370807846No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA1260.664416789706813No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC1010.5325880615903817No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA950.5009491668424383No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA900.47458342121915204No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA880.46403712296983757No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA860.4534908247205231No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA830.43767137734655137No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA830.43767137734655137No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA800.4218519299725796No Hit
GTGAATGGGGTTGCCAAAAAAAAAAAAAAAAAA770.40603248259860786No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA760.40075933347395065No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA750.3954861843492934No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA720.37966673697532166No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA710.3743935878506644No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA690.36384728960134993No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA690.36384728960134993No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG680.35857414047669267No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA620.3269352457287492No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA610.32166209660409195No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA610.32166209660409195No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA600.3163889474794347No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA580.3058426492301202No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA560.2952963509808057No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA540.28475005273149123No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA530.279476903606834No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA530.279476903606834No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA490.25838430710820504No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC480.2531111579835478No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC470.24783800885889054No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA470.24783800885889054No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC460.2425648597342333No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA450.23729171060957602No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA420.22147226323560432No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.22147226323560432No Hit
GTGAATGGGTACGTTGTTGCCAAAAAAAAAAAA400.2109259649862898No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA390.20565281586163256No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCAA380.20037966673697533No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA370.1951065176123181No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA370.1951065176123181No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA370.1951065176123181No Hit
ACGCAGAGTGAATGGGTTGTTGCCAAAAAAAAA370.1951065176123181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.18983336848766083No Hit
ACGCAGAGTGAATGGGGTTGTTGCCAAAAAAAA360.18983336848766083No Hit
GTTGTTGCCAAAAAAAAAAAAAAAAAAAAAAAA360.18983336848766083No Hit
ACGTACTCTAAAAAAAAAAAAAAAAAAAAAAAA350.1845602193630036No Hit
GTGAATGGGTTGCCAAAAAAAAAAAAAAAAAAA340.17928707023834634No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA330.17401392111368907No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT320.16874077198903184No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA320.16874077198903184No Hit
GAGTGAATGGGGTTGTTGCCAAAAAAAAAAAAA320.16874077198903184No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA310.1634676228643746No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.15819447373971735No Hit
ACGCAGAGTGAATGGGTGTTGCCAAAAAAAAAA300.15819447373971735No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA300.15819447373971735No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA290.1529213246150601No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA290.1529213246150601No Hit
ACGCAGAGTGAATGGGGTTGCCAAAAAAAAAAA290.1529213246150601No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA280.14764817549040285No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA280.14764817549040285No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA280.14764817549040285No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG270.14237502636574562No Hit
ACGTACTCTGAAAAAAAAAAAAAAAAAAAAAAA260.13710187724108838No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA260.13710187724108838No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA260.13710187724108838No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA250.13182872811643112No Hit
CAACGTACTCTAAAAAAAAAAAAAAAAAAAAAA250.13182872811643112No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA250.13182872811643112No Hit
GTTCGGCAACAACGTACTCTGCGTTTATACCAC240.1265555789917739No Hit
GTACGTTGTTGCCAAAAAAAAAAAAAAAAAAAA240.1265555789917739No Hit
ATGTTCGGCAACAACGTACTCTGCGTTGATACC230.12128242986711665No Hit
AACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA230.12128242986711665No Hit
GAGTACGTTGTTGCCAAAAAAAAAAAAAAAAAA220.11600928074245939No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11600928074245939No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11600928074245939No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA220.11600928074245939No Hit
ACGTACTCAAAAAAAAAAAAAAAAAAAAAAAAA220.11600928074245939No Hit
GAATGGGGTTGTTGCCAAAAAAAAAAAAAAAAA210.11073613161780216No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1054629824931449No Hit
AACGCAGAGTGAATGGGGTTGCCAAAAAAAAAA200.1054629824931449No Hit
GTGTTCGGCAAAAAAAAAAAAAAAAAAAAAAAA200.1054629824931449No Hit
ACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10018983336848766No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA190.10018983336848766No Hit
GAGTGAATGGGCAACAACGTACTCTGCGAAAAA190.10018983336848766No Hit
ACGCAGAGTGAATGGGTACGTTGTTGCCAAAAA190.10018983336848766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC350.00533759819.28571526
CTGCAAA653.7383514E-516.61538511
CTCTGCA603.9026668E-415.7500019
TCTGCAA603.9026668E-415.75000110
TGCAAAA700.001256223813.512
TATCAAC1006.7572873E-613.4999991
ATCAACG1203.154304E-612.3750012
ACGCAGA2850.012.3157891
CGCAGAG2850.012.3157892
TCTGCGT2800.012.05357227
GCAGAGT2950.011.8983053
TCAACGC1254.9809823E-611.883
CAGAGTG2800.011.5714294
AGAGTGA2800.011.5714295
CAACGCA1256.6750756E-510.84
AATGGGT1150.00459285279.3913054
AACGCAG1452.995552E-49.3103465
GGGCAAC2401.1382326E-79.00000114
TGGGCAA2401.1382326E-79.00000113
AATGGGG1350.00165596979.04
Produced by FastQC (version 0.11.2)