FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_83.3520000005bb66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_83.3520000005bb66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22743
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC2981.3102932770522797No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.8178340588312887No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA890.3913292001934661No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.3913292001934661No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.3429626698324759No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA660.29019918216594115No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA580.25502352372158466No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA490.21545090797168356No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA460.2022600360550499No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT440.19346612144396078No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA440.19346612144396078No Hit
GAGTACGTCACAAAAAAAAAAAAAAAAAAAAAA420.18467220683287164No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA410.1802752495273271No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA400.17587829222178253No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1670843776106934No Hit
GTCCTTCTGTGACGTACTCTGCGTTTATACCAC370.16268742030514882No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA370.16268742030514882No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA370.16268742030514882No Hit
GTGAATGGGAGTCCTAAAAAAAAAAAAAAAAAA300.1319087191663369No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA280.12311480455524776No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC270.1187178472497032No Hit
GTGAATGGGCAGTCCTAAAAAAAAAAAAAAAAA260.11432088994415865No Hit
GTGAATGGGAGTCCAAAAAAAAAAAAAAAAAAA240.10552697533306951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA1900.017.05263127
TATCAAC2050.014.4878041
ATCAACG2150.013.1860472
TCAACGC2100.012.8571423
GTATCAA1451.5816477E-712.1034491
TGCGTTG2950.010.52542426
CAACGCA2603.274181E-1110.3846154
TCTGCGT5350.010.09345827
CTGCGTT3353.6379788E-129.26865825
AACGCAG3303.255991E-108.5909095
CTCTGCG6400.08.437526
ACTCTGC6800.07.94117625
TACTCTG7050.07.46808524
GTACTCT7250.07.44827623
CGTACTC7450.07.24832222
ACGTACT7550.07.152317521
GACGTAC7550.07.152317520
TGTGACG7800.07.09615417
TGACGTA7650.07.058823619
CGCAGAG4401.5133992E-97.05681857
Produced by FastQC (version 0.11.2)