FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_80.3520000005bb09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_80.3520000005bb09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21603
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA3471.6062583900384206No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC2601.2035365458501135No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA2261.046150997546637No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA2181.0091191038281722No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA2150.9952321436837476No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA1900.8795074758135445No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA1730.8008147016618063No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.7591538212285331No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1320.611026246354673No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA1130.5230754987733185No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1100.5091885386288941No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA1090.5045595519140861No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.47678563162523724No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA990.4582696847660047No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA960.4443827246215803No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA920.4258667777623478No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA890.41197981761792346No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA880.4073508309031153No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA870.4027218441883072No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT840.38883488404388283No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA810.3749479238994584No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA750.3471740036106096No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC690.3194000833217609No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA690.3194000833217609No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA680.31477109660695274No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA660.3055131231773365No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA630.29162616303291206No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC610.28236818960329585No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA580.26848122945887143No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.25922325602925517No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC560.25922325602925517No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA540.24996528259963896No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA530.24533629588483083No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA510.23607832245521454No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA500.2314493357404064No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA490.22682034902559828No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA470.21756237559598207No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA470.21756237559598207No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA450.20830440216636578No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA440.20367541545155765No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA440.20367541545155765No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.19904642873674952No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA410.18978845530713329No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA410.18978845530713329No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA410.18978845530713329No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA410.18978845530713329No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC390.180530481877517No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA390.180530481877517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.180530481877517No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG390.180530481877517No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC390.180530481877517No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA370.17127250844790076No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA370.17127250844790076No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA360.16664352173309263No Hit
GTGAATGGGCTAACAAAAAAAAAAAAAAAAAAA340.15738554830347637No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA340.15738554830347637No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA340.15738554830347637No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA330.15275656158866824No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT330.15275656158866824No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA330.15275656158866824No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC320.1481275748738601No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1481275748738601No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA310.143498588159052No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.143498588159052No Hit
GAGTGAATGGGCTAACTATAAAAAAAAAAAAAA300.13886960144424387No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA300.13886960144424387No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA290.13424061472943571No Hit
CTAACTATCCACAAAAAAAAAAAAAAAAAAAAA290.13424061472943571No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA290.13424061472943571No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA290.13424061472943571No Hit
CCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12961162801462758No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA270.12498264129981948No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA270.12498264129981948No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC270.12498264129981948No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA260.12035365458501135No Hit
GTGAATGGGCTAACTATCAAAAAAAAAAAAAAA250.1157246678702032No Hit
ACGCAGAGTGAATGGGCTAACTATCAAAAAAAA250.1157246678702032No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA240.11109568115539507No Hit
GGGCTAACTATCCAAAAAAAAAAAAAAAAAAAA240.11109568115539507No Hit
TCAACGCAGAGTGAATGGGCTAACTATCCACAA230.10646669444058696No Hit
AACGCAGAGTGAATGGGCTAACTAAAAAAAAAA230.10646669444058696No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA230.10646669444058696No Hit
ACTATCCACGTACAAAAAAAAAAAAAAAAAAAA220.10183770772577883No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA220.10183770772577883No Hit
GAGTGAATGGGGCTAACTATCCAAAAAAAAAAA220.10183770772577883No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA220.10183770772577883No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA220.10183770772577883No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10183770772577883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG200.00842793127.02
ACGCTGA200.00842793127.01
GTATCAA853.0413503E-919.0588251
TATCAAC1507.2759576E-1114.41
TCAACGC2006.9703674E-910.7999993
ATCAACG2006.9703674E-910.7999992
TGCGTTG1955.7387297E-810.38461527
CAACGCA2152.1551386E-810.0465124
TCTGCGT3550.09.88732427
CTGCGTT2254.365029E-89.626
CAGAGTG4050.09.04
GCAGAGT3900.08.9999993
ACGCAGA3950.08.8860761
AGAGTGA4300.08.7906985
CGCAGAG4000.08.7752
AACGCAG2604.031299E-78.3076935
CTCTGCG4607.2759576E-127.63043526
GAGTGAA5650.07.407086
AGTGAAT5600.07.2321437
ACTCTGC4903.274181E-117.163265725
Produced by FastQC (version 0.11.2)