FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_8.3520000005b253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_8.3520000005b253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA1603.463203463203463No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA511.103896103896104No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA440.9523809523809524No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC410.8874458874458875No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA340.735930735930736No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA260.5627705627705628No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA230.49783549783549785No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA210.45454545454545453No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA210.45454545454545453No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA210.45454545454545453No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC200.4329004329004329No Hit
GGCACTGTACGTACAAAAAAAAAAAAAAAAAAA190.41125541125541126No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC130.2813852813852814No Hit
GGCATGAGCACTGTACGTACTCTGCGTTGATAC130.2813852813852814No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA120.2597402597402597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2380952380952381No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA110.2380952380952381No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA90.19480519480519481No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA90.19480519480519481No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA90.19480519480519481No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA90.19480519480519481No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT90.19480519480519481No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA80.17316017316017315No Hit
TATCAACGCAGAGTGAATGGGCATGAGCAAAAA80.17316017316017315No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA80.17316017316017315No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG80.17316017316017315No Hit
ACGCAGAGTGAATGGGCATGAGCAAAAAAAAAA80.17316017316017315No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA80.17316017316017315No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC80.17316017316017315No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15151515151515152No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA70.15151515151515152No Hit
ACGCAGAGTGAATGGGCACTGTACAAAAAAAAA70.15151515151515152No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA70.15151515151515152No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA70.15151515151515152No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA70.15151515151515152No Hit
CCCCCCCATGAGCACTGTACGTACTCTGCGTTG70.15151515151515152No Hit
GAATGGGCACTGTACGTAAAAAAAAAAAAAAAA70.15151515151515152No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15151515151515152No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA70.15151515151515152No Hit
GCACTGTACGTACTCTGAAAAAAAAAAAAAAAA60.12987012987012986No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA60.12987012987012986No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA60.12987012987012986No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA60.12987012987012986No Hit
GTGAATGGGCATGAGCAAAAAAAAAAAAAAAAA60.12987012987012986No Hit
TATCAACGCAGAGTGAATGGGCATGAGCACAAA60.12987012987012986No Hit
GGCACTGTACGTACTCAAAAAAAAAAAAAAAAA60.12987012987012986No Hit
TATCAACGCAGAGTGAATGGGCACTTTACGTAC60.12987012987012986No Hit
CGCATGAGCACTGTACGTACTCTGCGTTGATAC60.12987012987012986No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA60.12987012987012986No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC60.12987012987012986No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA50.10822510822510822No Hit
GCGTTGATACCACTGCTTGTATCAACGCAGAGT50.10822510822510822No Hit
GTATCAACGCAGAGTGAATGGGCATGAGCAAAA50.10822510822510822No Hit
GCATGAGCACAAAAAAAAAAAAAAAAAAAAAAA50.10822510822510822No Hit
GAATGGGCACTGTACGAAAAAAAAAAAAAAAAA50.10822510822510822No Hit
TATCAACGCAGAGTGAATGGGCACTGTACAAAA50.10822510822510822No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC50.10822510822510822No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC50.10822510822510822No Hit
GGCATGAGCAAAAAAAAAAAAAAAAAAAAAAAA50.10822510822510822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGGG550.003449073514.7272726
CAGAGTG600.00615515613.54
AGAGTGA600.00615515613.55
GTGAATG1150.00356408819.3913041
TGAATGG1150.00356408819.3913042
TCTGCGT1308.536403E-49.34615327
GAATGGG1200.0050725479.03
CTCTGCG1700.0098119947.14705926
Produced by FastQC (version 0.11.2)