FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_78.3520000005bac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_78.3520000005bac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14884
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA1751.1757592045149154No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1430.9607632356893308No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1350.9070142434829348No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1330.8935769954313357No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1110.7457672668637463No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA920.6181134103735555No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA710.4770223058317657No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA650.43671056167696853No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA590.3963988175221715No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT580.38968019349637195No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA560.37624294544477294No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA550.3695243214189734No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA530.35608707336737433No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA440.29561945713517873No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA430.2889008331093792No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.2620263370061811No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.2149959688255845No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA300.20155872077398548No Hit
GAGTGAATGGGGTTCGTCAAAAAAAAAAAAAAA280.18812147272238647No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA270.18140284869658693No Hit
ACGCAGAGTGAATGGGTTCGTCACAAAAAAAAA270.18140284869658693No Hit
AACGCAGAGTGAATGGGTTCGTCAAAAAAAAAA270.18140284869658693No Hit
ACGCAGAGTGAATGGGGTTCGTCAAAAAAAAAA270.18140284869658693No Hit
GAGTGAATGGGTTCGTCAAAAAAAAAAAAAAAA250.16796560064498792No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA250.16796560064498792No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA250.16796560064498792No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCAAAAA230.15452835259338887No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT220.14780972856758937No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA220.14780972856758937No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA210.14109110454178983No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA210.14109110454178983No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA210.14109110454178983No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA210.14109110454178983No Hit
ACGCAGAGTGAATGGGTTCGTCACACAAAAAAA200.13437248051599032No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC190.12765385649019081No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA190.12765385649019081No Hit
GAATGGGGTTCGTCAAAAAAAAAAAAAAAAAAA180.1209352324643913No Hit
GTGAATGGGGTTCGTCACAAAAAAAAAAAAAAA180.1209352324643913No Hit
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA170.11421660843859177No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA170.11421660843859177No Hit
TTCGTCACACTACGTACTCTGCGTTGATACCAC170.11421660843859177No Hit
AACGCAGAGTGAATGGGGTTCGTCAAAAAAAAA160.10749798441279225No Hit
AATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA160.10749798441279225No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC150.10077936038699274No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA150.10077936038699274No Hit
GTGAATGGGAGTTCGTCAAAAAAAAAAAAAAAA150.10077936038699274No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACT150.10077936038699274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT451.5177447E-624.023
GTTGATA401.699208E-523.62524
GATACCA401.699208E-523.62527
TGATACC401.699208E-523.62526
TTGATAC401.699208E-523.62525
TGTTCGT706.889052E-515.4285728
GCGTTGA706.889052E-515.42857222
TCTGCGT2300.014.08695627
TATCAAC903.7433554E-513.51
TCAACGC853.5899007E-412.7058813
ATCAACG853.5899007E-412.7058812
TGCGTTG905.814511E-412.021
AATGGGA1051.5866413E-411.5714284
AATGGGT1102.445877E-411.0454554
CTCTGCG3150.010.28571526
CTGCGTT1050.002114025610.28571420
GTGAATG4850.010.0206181
TGAATGG4850.010.0206182
CAACGCA1100.00311152439.8181824
ACTCTGC3450.09.39130525
Produced by FastQC (version 0.11.2)