FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_77.3520000005baab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_77.3520000005baab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16923
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA3371.991372688057673No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC2281.3472788512675058No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA2051.211369142587012No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA910.5377297169532589No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA890.5259114814158247No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA770.4550020681912191No Hit
ACGCAGAGTGAATGGGAGGTCCAAAAAAAAAAA740.43727471488506764No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.42545647934763337No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA630.3722744194291792No Hit
GTGAATGGGTGCTTGGACAAAAAAAAAAAAAAA560.33091059504815934No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA550.3250014772794422No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA530.3131832417420079No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA530.3131832417420079No Hit
TATCAACGCAGAGTGAATGGGAGGTCCAAAAAA520.3072741239732908No Hit
ACGCAGAGTGAATGGGGTCCAAAAAAAAAAAAA520.3072741239732908No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA490.2895467706671394No Hit
GTGAATGGGGTACGTAAGGTCCAAAAAAAAAAA470.27772853512970513No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA470.27772853512970513No Hit
GTGAATGGGTGCTTGGACCAAAAAAAAAAAAAA460.271819417360988No Hit
AACGCAGAGTGAATGGGAGGTCCAAAAAAAAAA440.26000118182355375No Hit
ACGCAGAGTGAATGGGTACGTAAGGTCCAAAAA440.26000118182355375No Hit
GTGAATGGGAAGGTCCAAAAAAAAAAAAAAAAA440.26000118182355375No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA410.24227382851740234No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA410.24227382851740234No Hit
GAGTGAATGGGGGTCCAAAAAAAAAAAAAAAAA390.2304555929799681No Hit
GCTTGGACCTAAAAAAAAAAAAAAAAAAAAAAA350.20681912190509957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.20091000413638244No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.20091000413638244No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA330.1950008863676653No Hit
ACGCAGAGTGAATGGGGGTCCAAAAAAAAAAAA330.1950008863676653No Hit
ACGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAA320.1890917685989482No Hit
GAGTGAATGGGAGGTCCAAAAAAAAAAAAAAAA320.1890917685989482No Hit
TATCAACGCAGAGTGAATGGGGTCCAAAAAAAA320.1890917685989482No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA300.17727353306151392No Hit
ACGCAGAGTGAATGGGGAGTACGTAAGGTCCAA300.17727353306151392No Hit
GGGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA290.17136441529279678No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA290.17136441529279678No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA280.16545529752407967No Hit
GAATGGGAGGTCCAAAAAAAAAAAAAAAAAAAA280.16545529752407967No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC280.16545529752407967No Hit
TGCTTGGACAAAAAAAAAAAAAAAAAAAAAAAA280.16545529752407967No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA260.1536370619866454No Hit
AACGCAGAGTGAATGGGGTCCAAAAAAAAAAAA260.1536370619866454No Hit
TGCTTGGACCTAAAAAAAAAAAAAAAAAAAAAA250.1477279442179283No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1477279442179283No Hit
GTGCTTGGACCTTACGTACTCTGCGTTGATACC250.1477279442179283No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA250.1477279442179283No Hit
GGTACGTAAGGTCCAAAAAAAAAAAAAAAAAAA250.1477279442179283No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA240.14181882644921112No Hit
GCTTGGACCTTACGTACTCTGCGTTTATACCAC240.14181882644921112No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA240.14181882644921112No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA240.14181882644921112No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT240.14181882644921112No Hit
ACGCAGAGTGAATGGGAAGGTCCAAAAAAAAAA230.135909708680494No Hit
GTATCAACGCAGAGTGAATGGGAGGTCCAAAAA230.135909708680494No Hit
GTAAGGTCCAAAAAAAAAAAAAAAAAAAAAAAA230.135909708680494No Hit
GAATGGGGAGTACGTAAGGTCCAAAAAAAAAAA230.135909708680494No Hit
CTTGGACCTTACGTACTCTGCGTTGATACCACT230.135909708680494No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA220.13000059091177688No Hit
GTGAATGGGTGCTTGGAAAAAAAAAAAAAAAAA220.13000059091177688No Hit
ACGCAGAGTGAATGGGGTACGTAAGGTCCAAAA220.13000059091177688No Hit
CTGCTTGGACCTTACGTACTCTGCGTTGATACC220.13000059091177688No Hit
ATCAACGCAGAGTGAATGGGAGGTCCAAAAAAA210.12409147314305974No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA210.12409147314305974No Hit
GTGAATGGGATGCTTGGACCTAAAAAAAAAAAA210.12409147314305974No Hit
GGGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAA200.11818235537434262No Hit
CGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAAA200.11818235537434262No Hit
ACGCAGAGTGAATGGGTAAGGTCCAAAAAAAAA200.11818235537434262No Hit
GAGTGAATGGGATGCTTGGACCAAAAAAAAAAA200.11818235537434262No Hit
GACGTAAGGTCCAAAAAAAAAAAAAAAAAAAAA200.11818235537434262No Hit
CAACGCAGAGTGAATGGGAGGTCCAAAAAAAAA190.11227323760562546No Hit
AACGCAGAGTGAATGGGGGTCCAAAAAAAAAAA190.11227323760562546No Hit
GAGTGAATGGGGTACGTAAGGTCCAAAAAAAAA190.11227323760562546No Hit
TATCAACGCAGAGTGAATGGGGGTCCAAAAAAA190.11227323760562546No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA180.10636411983690834No Hit
GTGAATGGGTGCTTGGACCTAAAAAAAAAAAAA180.10636411983690834No Hit
GAGTGAATGGGGTCCAAGCAAAAAAAAAAAAAA180.10636411983690834No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA180.10636411983690834No Hit
GTGAATGGGATGCTTGGACCAAAAAAAAAAAAA180.10636411983690834No Hit
GTATCAACGCAGAGTGAATGGGATGCTTGGACC180.10636411983690834No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.10045500206819122No Hit
GAGTGAATGGGATGCTTGGACCTAAAAAAAAAA170.10045500206819122No Hit
ACGCAGAGTGAATGGGTGCTTGGACCAAAAAAA170.10045500206819122No Hit
GATACCACTGCTTGGACCTTACGTACTCTGCGT170.10045500206819122No Hit
ATCAACGCAGAGTGAATGGGGTCCAAAAAAAAA170.10045500206819122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00835357927.0148351
TATCAAC1307.2759576E-1216.6245161
ATCAACG1351.2732926E-1116.0087932
TCAACGC1402.1827873E-1115.4370493
CAACGCA1551.1277734E-1013.9431424
GTATCAA700.001241279113.5074181
GATACCA750.002087801212.60692327
TGATACC850.005330800511.12375626
TTGCTTG850.005451034311.0908468
TCTGCGT3950.010.94271927
TTGATAC900.00815660210.5057725
CTCTGCG4400.09.82357726
TGCGTTG1555.275026E-59.5859127
AACGCAG2401.02045306E-89.5677551
CTGCTTG2358.2480256E-89.1693328
ATGGGGT1400.00233058218.657657
ACTCTGC5050.08.55915625
TACTCTG5050.08.55915624
TGGACCT5350.08.33167813
GACCTTA5350.08.33167815
Produced by FastQC (version 0.11.2)