FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_76.3520000005ba81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_76.3520000005ba81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14403
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA1881.30528362146775No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1420.9859057140873428No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1170.8123307644240784No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA1120.7776157744914254No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT1000.6942997986530584No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA990.6873568006665278No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA870.6040408248281608No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA830.5762688328820385No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA720.49989585503020206No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT660.45823786711101855No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA610.42352287717836556No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA600.41657987919183503No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA550.3818648892591821No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA540.3749218912726515No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA520.36103589529959035No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA460.3193779073804069No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA430.29854891342081513No Hit
ACGCAGAGTGAATGGGATCTCGTCAAAAAAAAA390.2707769214746928No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA380.2638339234881622No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA370.2568909255016316No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA350.24300492952857045No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA330.22911893355550927No Hit
GAATGGGATCTCGTCCAAAAAAAAAAAAAAAAA320.2221759355689787No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA320.2221759355689787No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.2152329375824481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.20134694160938693No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC290.20134694160938693No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT270.18746094563632576No Hit
CGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.18051794764979517No Hit
CGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.18051794764979517No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA260.18051794764979517No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1735749496632646No Hit
ACGCAGAGTGAATGGGATCTCGTCCTCAAAAAA240.166631951676734No Hit
GTGAATGGGGATCTCGTCAAAAAAAAAAAAAAA240.166631951676734No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCAA230.15968895369020344No Hit
GGTACGTAGAGGACGAAAAAAAAAAAAAAAAAA230.15968895369020344No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA220.15274595570367286No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA220.15274595570367286No Hit
GATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAA210.14580295771714227No Hit
ATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAAA210.14580295771714227No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC210.14580295771714227No Hit
GTGAATGGGGATCTCGTCCAAAAAAAAAAAAAA200.1388599597306117No Hit
GTGAATGGGATCTCGTCCTAAAAAAAAAAAAAA190.1319169617440811No Hit
AACGCAGAGTGAATGGGATCTCGTCCTCAAAAA190.1319169617440811No Hit
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA190.1319169617440811No Hit
GATCTCGTCAAAAAAAAAAAAAAAAAAAAAAAA190.1319169617440811No Hit
GTACGTAGAGGACGAAAAAAAAAAAAAAAAAAA190.1319169617440811No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC180.12497396375755052No Hit
ATCAACGCAGAGTGAATGGGATCTCGTCCAAAA180.12497396375755052No Hit
GAATGGGATCTCGTCAAAAAAAAAAAAAAAAAA180.12497396375755052No Hit
ATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAAA170.11803096577101993No Hit
GTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11803096577101993No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA160.11108796778448934No Hit
GTGAATGGGGTACGTAGAGGACGAAAAAAAAAA160.11108796778448934No Hit
GTGAATGGGGATCTCGAAAAAAAAAAAAAAAAA150.10414496979795876No Hit
GTGAATGGGCATCTCGTCCTCAAAAAAAAAAAA150.10414496979795876No Hit
ATCAACGCAGAGTGAATGGGATCTCGTCCTCAA150.10414496979795876No Hit
ATGGGATCTCGTCCAAAAAAAAAAAAAAAAAAA150.10414496979795876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1353.5561243E-914.01
ATCAACG1552.4761903E-812.1935482
TCAACGC1603.8577127E-811.81253
CTGCGTT2201.8553692E-1011.04545526
TGCGTTG2101.0622898E-910.92857227
CAACGCA1657.649196E-710.6363644
AGAGTGA3250.09.9692325
CAGAGTG3201.8189894E-129.7031254
GCAGAGT3201.2732926E-119.281253
CGCAGAG3302.5465852E-119.02
ACGCAGA3353.45608E-118.8656711
GAGTGAA3951.8189894E-128.5443046
AGTGAAT3951.8189894E-128.5443047
TCTGCGT3201.380613E-98.437527
AACGCAG2152.2405771E-58.162795
CTCTGCG3507.4451236E-97.714285426
AATGGGA3601.2596502E-87.54
GTATCAA1850.00324344967.2972971
TGAATGG5550.07.2972972
GTGAATG5600.07.2321431
Produced by FastQC (version 0.11.2)